Effect of TGF-β1 Stimulation on the Smad Signal Transduction Pathway of Human Peritoneal Mesothelial Cells
| العنوان: | Effect of TGF-β1 Stimulation on the Smad Signal Transduction Pathway of Human Peritoneal Mesothelial Cells |
|---|---|
| المؤلفون: | Zhang, Hao, Liu, Fu-you, Liu, Ying-hong, Peng, You-ming, Liao, Qin, Zhang, Ke |
| المصدر: | International Journal of Biomedical Science : IJBS |
| بيانات النشر: | Master Publishing Group, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | mesothelial cells, Smad7, TGF-β1, extracellular matrix, Smad2/3, Article |
| الوصف: | Objective: To prove whether the SMAD signal transduction pathway in human peritoneal mesothelial cells (HPMCs) influenced the process of human peritoneal fibrosis stimulated by TGF-β1. Methods: HPMCs were isolated from normal human omentum and the third generation cells were stimulated by 5 ng/ml TGF-β1. Immunohistochemistry, Western blotting, ELISA and RT-PCR were employed to investigate the protein expression of p-Smad2/3 and the protein and mRNA expressions of SMAD 7, fibronectin (FN) and collagen-I (COL1). Results: The protein expression of p-Smad2/3 in HPMCs was remarkably increased 15 min (29% p-Smad2/3-positive cells) after TGF-β1 stimulation, peaking from 30 min (81%) to 1 h (84%) and dropping after 2 h (37%); Meanwhile, p-Smad2/3 mainly distributed in cytoplasm at 15 min, concentrated in cell nucleus and peri-nucleus from 30 min to 1 h, and distributed in cytoplasm again at 2 h. The protein expression of SMAD 7 in cells was obviously increased 24 h after TGF-β1 stimulation, peaking at 48 h. The mRNA expression of SMAD7 was time-dependently increased. The expressions of extracellular FN protein, intracellular FN mRNA, as well as intracellular COL1 protein and mRNA were significantly increased and all of them displayed time dependency. Conclusions: The SMAD signal transduction pathway of HPMCs can be specifically activated by TGF-β1 and influence the process of human peritoneal fibrosis. The protein and mRNA expression of SMAD 7 (an inhibitor of SMAD pathway) are significantly increased as a result of feedback. |
| اللغة: | English |
| تدمد: | 1555-2810 1550-9702 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid________::74d9e5f6e6370ab8f50f1baa3ff54ac6 http://europepmc.org/articles/PMC3614575 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.pmid..........74d9e5f6e6370ab8f50f1baa3ff54ac6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15552810 15509702 |
|---|