Neutrophil-epithelial interactions augment infectivity and pro-inflammatory responses to SARS-CoV-2 infection
| العنوان: | Neutrophil-epithelial interactions augment infectivity and pro-inflammatory responses to SARS-CoV-2 infection |
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| المؤلفون: | Calvert, BA, Quiroz, EJ, Lorenzana, Z, Doan, N, Kim, S, Senger, CN, Wallace, WD, Salomon, MP, Henley, JE, Ryan, AL |
| المصدر: | bioRxiv article-version (status) pre article-version (number) 1 |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Inflammation, Airway Epithelium, Cell-Cell Interactions, Neutrophils, Viral Infection, COVID-19, Cytokines, Interferon, respiratory system, Article, respiratory tract diseases, Tissue Remodeling |
| الوصف: | In response to viral infection, neutrophils release inflammatory mediators as part of the innate immune response, contributing to pathogen clearance through virus internalization and killing. Pre-existing co- morbidities correlating to incidence of severe COVID-19 are associated with chronic airway neutrophilia. Furthermore, examination of COVID-19 explanted lung tissue revealed a series of epithelial pathologies associated with the infiltration and activation of neutrophils, indicating neutrophil activity in response to SARS- CoV-2 infection. To determine the impact of neutrophil-epithelial interactions on the infectivity and inflammatory responses to SARS-CoV-2 infection, we developed a co-culture model of airway neutrophilia. SARS-CoV-2 infection of the airway epithelium alone does not result in a notable pro-inflammatory response from the epithelium. The addition of neutrophils induces the release of proinflammatory cytokines and stimulates a significantly augmented pro-inflammatory response subsequent SARS-CoV-2 infection. The resulting inflammatory response is polarized with differential release from the apical and basolateral side of the epithelium. Additionally, the integrity of the epithelial barrier is impaired with notable epithelial damage and infection of basal stem cells. This study reveals a key role for neutrophil-epithelial interactions in determining inflammation and infectivity in response to SARS-CoV-2 infection. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid________::8d433e08b140a110079ec1040a0dc3cc http://europepmc.org/articles/PMC8366793 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.pmid..........8d433e08b140a110079ec1040a0dc3cc |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |