Lake Hongze is an essential storage lake for the "Eastern Route of the South-North Water Diversion Project" and plays an important role in climate adjustment and flood prevention and control. To study the structural evolution of phytoplankton communities in Lake Hongze and their relationship with environmental factors and to understand the status of the ecosystem of Lake Hongze before the "ten-year fishing ban," monthly monitoring was carried out from 2015 to 2020. During the study period, total nitrogen showed a significant downward trend starting in 2017, the total phosphorus and chemical oxygen demand declined slightly, the water temperature had no obvious change trend, and the water depth and transparency increased from 2015 to 2018 and then declined significantly. A total of 102 genera of phytoplankton in eight phyla were identified, with a total of 310 species. The dominant phytoplankton phyla mainly included Chlorophyta and Bacillariophyta, followed by Cyanophyta and Pyrrophyta. The dominant genera were