| الوصف: |
To investigate the effect of Zhibai Dihuang Decoction (ZDD) on the sperm mitochondrial respiratory chain complex (MRCC) in rats with Ureaplasma urealyticum (UU) infection.Ninety male SD rats were randomly divided into five groups, sham operation, UU infection model control, ZDD (crude drug at 8.56 g per kg of the body weight per day), doxycycline (DC, at 20 mg per kg of the body weight per day), and ZDD+DC. The model of UU infection was established by injecting UU into the bladder of all the rats except those of the sham operation group. After modeling, the rats were treated intragastrically with respective drugs for 21 days and then executed and their epididymides harvested for examination of sperm quality and determination of the activities of sperm MRCCs I, II, III and IV by spectrophotometry.At 10 days after modeling, the UU-positive rates in the model control, sham operation, ZDD, DC and ZDD+DC groups were 92.9%, 0%, 33.3%, 26.7% and 20.0%, respectively, significantly higher in the model control than in the other groups (P0.05). The epididymal sperm concentrations in the five groups were (0.97±0.23), (3.02±0.52), (1.21±0.35), (1.02±0.31) and (1.52±0.28) ×106 ml, the sperm motilities were (58.62±15.36), (80.45±7.21), (75.52±8.78), (68.43±10.25) and (78.25±7.67)%, and rates of grade a+b sperm were (6.15±1.02), (10.32±1.14), (10.12±1.08), (9.01+1.27) and (10.74±1.03)%, respectively, all remarkably lower in the model control than in the sham operation group (P0.01), but markedly higher in the ZDD and ZDD+DC groups than in the model controls (P0.05). The activities of MRCC I in the model control, sham operation, ZDD, DC and ZDD+DC groups were (31.54±16.25), (136.86±6.34), (100.68±14.41), (81.68±6.78) and (124.06±5.54) μmol/(min·mg), those of MRCC II were (9.50±3.86), (20.34±0.37), (10.88±1.04), (12.93±1.07) and (16.23±0.60) μmol/(min·mg), those of MRCC III were (5.58±1.79), (19.60±0.61), (11.34±1.35), (13.87±1.23) and (15.96±0.69) μmol/(min·mg), and those of MRCC IV were (9.54±1.34), (28.98±3.33), (17.02±2.04), (18.41±2.67) and (21.66±2.93) μmol/(min·mg), respectively, all significantly lower in the model control than in the sham operation group (P0.01), with the activities of MRCCs I, III and IV remarkably higher in the ZDD, DC and ZDD+DC groups (P0.01) and that of MRCC II higher in the DC and ZDD+DC groups than in the model control (P0.05).ZDD can improve the epididymal sperm quality and the activity of the sperm MRCC in UU-infected rats, which may be one of the mechanisms of ZDD acting on male infertility caused by UU infection.目的: 观察知柏地黄汤对解脲脲原体（UU）感染模型大鼠精子线粒体呼吸链复合物的影响。方法: 90只雄性SD大鼠，随机分成空白对照组、模型对照组、中药组(知柏地黄汤8.56 g生药/（kg·d)）、西药组［强力霉素20 mg/（kg·d)］、中西药组（知柏地黄汤+强力霉素等效剂量之和），除空白对照组外，其他组均采用经大鼠膀胱注射解脲脲原体建立UU感染动物模型，然后，连续灌胃21 d(各组给予对应药物)后处死，取附睾标本检测精子质量，分光光度法检测精子线粒体呼吸链复合物（Ⅰ、Ⅱ、Ⅲ、Ⅳ）活性。结果: ①模型对照组、空白对照组、中药组、西药组、中西药组建模10 d后UU培养阳性率分别为92.9%、0%、33.3%、26.7%、20.0%，模型对照组UU阳性率明显高于其他治疗组（P0.05）。②模型对照组、空白对照组、中药组、西药组、中西药组大鼠附睾精子浓度分别为（0.97±0.23）、（3.02±0.52）、（1.21±0.35）、（1.02±0.31）、（1.52±0.28）×10⁶/ml，活动率分别为（58.62±15.36）%、(80.45±7.21)%、(75.52±8.78)%、(68.43±10.25)%、(78.25±7.67)%，a+b级精子百分率分别为（6.15±1.02）%、（10.32±1.14）%、（10.12±1.08）%、（9.01±1.27）%、（10.74±1.03）%；模型对照组大鼠附睾精子浓度、活动率及a+b级精子百分率明显低于空白对照组（P0.01）；各治疗组大鼠附睾精子活动率及a+b级精子百分率明显高于模型对照组（P0.01）。③模型对照组、空白对照组、中药组、西药组、中西药组大鼠精子线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ活性分别为（31.54±16.25）、（136.86±6.34）、（100.68±14.41）、（81.68±6.78）、（124.06±5.54） μmol/(min·mg)，（9.50±3.86）、（20.34±0.37）、（10.88±1.04）、（12.93±1.07）、（16.23±0.60） μmol/(min·mg)，（5.58±1.79）、（19.60±0.61）、（11.34±1.35）、（13.87±1.23）、（15.96±0.69） μmol/(min·mg)，（9.54±1.34）、（28.98±3.33）、（17.02±2.04）、（18.41±2.67）、（21.66±2.93） μmol/(min·mg)。模型对照组大鼠精子线粒体呼吸链复合物Ⅰ、Ⅱ、Ⅲ、Ⅳ活性明显低于空白对照组（P0.01）；中药组、西药组、中西药组大鼠精子线粒体呼吸链复合物Ⅰ、Ⅲ、Ⅳ活性明显高于模型对照组（P0.01），西药组、中西药组大鼠精子线粒体呼吸链复合物Ⅱ活性高于模型对照组（P0.05）。结论: 知柏地黄汤能够提高UU感染大鼠精子质量及线粒体呼吸链复合物活性。这可能是知柏地黄汤治疗UU感染所致不育症的机制之一。. |
