To analyze the characteristics of long non-coding RNA (lncRNA) expression in non-alcoholic fatty liver disease (NAFLD). Methods: lncRNA-mRNA microarray was conducted on the liver tissue samples from 10 patients with simple gallbladder stone (5 NAFLD liver samples and 5 normal liver samples), and the differentially expressed lncRNA was analyzed by bioinformatics technology. Results: Compared with the normal liver samples, there were abnormal expression of 1 735 lncRNAs and 1 485 mRNAs in NAFLD liver samples. Among them, 535 lncRNAs and 760 mRNAs were up-regulated, 1 200 lncRNAs and 725 mRNAs were down-regulated. Conclusion: Compared with normal liver, the expression of lncRNA in NAFLD tissues is obviously abnormal. These lncRNAs may play an important role in the occurrence and development of NAFLD.目的：分析非酒精性脂肪肝病(non-alcoholic fatty liver disease，NAFLD)中长链非编码RNA(lncRNA)的表达谱特征。方法：采用lncRNA-mRNA基因芯片检测单纯性胆囊结石伴或不伴NAFLD患者的肝组织样本，获得lncRNA差异性表达谱。进一步使用生物信息学技术对差异表达的基因予以分析。结果：与正常样本相比，NAFLD肝样本内共有 1 735个lncRNAs和1 485个mRNAs异常表达，其中535个lncRNAs和760个mRNAs上调，1 200个lncRNAs和725个mRNAs下调。结论：与正常肝组织相比，NAFLD组织中lncRNA表达谱明显异常，这些lncRNAs可能在NAFLD的发生和发展中发挥重要作用。.
