Simultaneous monitoring of potassium, glucose and lactate during spreading depolarization in the injured human brain – Proof of principle of a novel real-time neurochemical analysis system, continuous online microdialysis
| العنوان: | Simultaneous monitoring of potassium, glucose and lactate during spreading depolarization in the injured human brain – Proof of principle of a novel real-time neurochemical analysis system, continuous online microdialysis |
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| المؤلفون: | Rogers, Michelle L, Leong, Chi Leng, Gowers, Sally AN, Samper, Isabelle C, Jewell, Sharon L, Khan, Asma, McCarthy, Leanne, Pahl, Clemens, Tolias, Christos M, Walsh, Daniel C, Strong, Anthony J, Boutelle, Martyn G |
| المصدر: | Journal of Cerebral Blood Flow & Metabolism |
| بيانات النشر: | SAGE Publications, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | On-line microdialysis, ischaemic brain injury, Microdialysis, Cortical Spreading Depression, microfluidics, Original Articles, Neurophysiological Monitoring, Online Systems, Glucose, spreading depolarization, Brain Injuries, Clinical Studies, Potassium, Humans, Electrocorticography, Lactic Acid, Coma, neurometabolic coupling |
| الوصف: | Spreading depolarizations occur spontaneously and frequently in injured human brain. They propagate slowly through injured tissue often cycling around a local area of damage. Tissue recovery after an spreading depolarization requires greatly augmented energy utilisation to normalise ionic gradients from a virtually complete loss of membrane potential. In the injured brain, this is difficult because local blood flow is often low and unreactive. In this study, we use a new variant of microdialysis, continuous on-line microdialysis, to observe the effects of spreading depolarizations on brain metabolism. The neurochemical changes are dynamic and take place on the timescale of the passage of an spreading depolarization past the microdialysis probe. Dialysate potassium levels provide an ionic correlate of cellular depolarization and show a clear transient increase. Dialysate glucose levels reflect a balance between local tissue glucose supply and utilisation. These show a clear transient decrease of variable magnitude and duration. Dialysate lactate levels indicate non-oxidative metabolism of glucose and show a transient increase. Preliminary data suggest that the transient changes recover more slowly after the passage of a sequence of multiple spreading depolarizations giving rise to a decrease in basal dialysate glucose and an increase in basal dialysate potassium and lactate levels. |
| اللغة: | English |
| تدمد: | 1559-7016 0271-678X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid________::bb573822907cbaf50fd689e80d4f610c http://europepmc.org/articles/PMC5414898 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.pmid..........bb573822907cbaf50fd689e80d4f610c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15597016 0271678X |
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