Class I HDACs share a common mechanism of regulation by inositol phosphates
| العنوان: | Class I HDACs share a common mechanism of regulation by inositol phosphates |
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| المؤلفون: | Millard, CJ, Watson, PJ, Celardo, I, Gordiyenko, Y, Cowley, SM, Robinson, CV, Fairall, L, Schwabe, JW |
| المصدر: | Molecular Cell |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Models, Molecular, Protein Folding, Inositol Phosphates, Molecular Sequence Data, Histone Deacetylase 1, Cell Biology, Article, Histone Deacetylases, Protein Structure, Tertiary, Substrate Specificity, Repressor Proteins, HEK293 Cells, Trans-Activators, Humans, Amino Acid Sequence, Dimerization, Molecular Biology |
| الوصف: | Summary Class I histone deacetylases (HDAC1, HDAC2, and HDAC3) are recruited by cognate corepressor proteins into specific transcriptional repression complexes that target HDAC activity to chromatin resulting in chromatin condensation and transcriptional silencing. We previously reported the structure of HDAC3 in complex with the SMRT corepressor. This structure revealed the presence of inositol-tetraphosphate [Ins(1,4,5,6)P4] at the interface of the two proteins. It was previously unclear whether the role of Ins(1,4,5,6)P4 is to act as a structural cofactor or a regulator of HDAC3 activity. Here we report the structure of HDAC1 in complex with MTA1 from the NuRD complex. The ELM2-SANT domains from MTA1 wrap completely around HDAC1 occupying both sides of the active site such that the adjacent BAH domain is ideally positioned to recruit nucleosomes to the active site of the enzyme. Functional assays of both the HDAC1 and HDAC3 complexes reveal that Ins(1,4,5,6)P4 is a bona fide conserved regulator of class I HDAC complexes. Graphical Abstract Highlights • Inositol phosphates are bona fide regulators of class I HDAC corepressor complexes • The ELM2-SANT motif is a conserved HDAC corepressor assembly module • MTA1 is a dimer that recruits two HDACs into the NuRD complex • The MTA1-BAH domain is positioned to recruit chromatin to the HDAC active site |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::11698968cfafaef0db3075b37517dd2a https://ora.ox.ac.uk/objects/uuid:db744068-d8c9-4f58-8d5a-4c0996a4f636 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.pmid.dedup....11698968cfafaef0db3075b37517dd2a |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |