Chromosome Conformation Capture on Chip (4C): Data Processing
| العنوان: | Chromosome Conformation Capture on Chip (4C): Data Processing |
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| المؤلفون: | Leblanc , B, Comet , I, Bantignies , F, cavalli , G |
| المساهمون: | Institut de génétique humaine ( IGH ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), BRIC - University of Copenhagen, Ole Maaløes Vej 5, DK-2200, Copenhagen N, Denmark, Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Biotech Research and Innovation Centre (BRIC), University of Copenhagen = Københavns Universitet (KU) |
| المصدر: | Methods in Molecular Biology (Clifton then Totova) Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2016, 1480, pp.243-261 Methods in Molecular Biology Methods in Molecular Biology, Humana Press/Springer Imprint, 2016, 1480, pp.243-261. ⟨10.1007/978-1-4939-6380-5_21⟩ |
| بيانات النشر: | HAL CCSD, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Chromatin Immunoprecipitation, [SDV.GEN]Life Sciences [q-bio]/Genetics, [ SDV ] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], Chromosome Mapping, Microarray Analysis, Chromatin, Chromosomes, Histones, Mice, Animals, Nucleic Acid Conformation, Drosophila, [ SDV.GEN ] Life Sciences [q-bio]/Genetics, ComputingMilieux_MISCELLANEOUS |
| الوصف: | 4C methods are useful to investigate dependencies between regulatory mechanisms and chromatin structures by revealing the frequency of chromatin contacts between a locus of interest and remote sequences on the chromosome. In this chapter we describe a protocol for the data analysis of microarray-based 4C experiments, presenting updated versions of the methods we used in a previous study of the large-scale chromatin interaction profile of a Polycomb response element in Drosophila. The protocol covers data preparation, normalization, microarray probe selection, and the multi-resolution detection of regions with enriched chromatin contacts. A reanalysis of two independent mouse datasets illustrates the versatility of this protocol and the importance of data processing in 4C. Methods were implemented in the R package MRA.TA (Multi-Resolution Analyses on Tiling Array data), and they can be used to analyze ChIP-on-chip data on broadly distributed chromatin components such as histone marks. |
| اللغة: | English |
| تدمد: | 1064-3745 1940-6029 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::2f7dc4c2a672578e2fdb0f9167130253 https://hal.archives-ouvertes.fr/hal-01377541 |
| رقم الأكسشن: | edsair.pmid.dedup....2f7dc4c2a672578e2fdb0f9167130253 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10643745 19406029 |
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