Systematic tracking of altered haematopoiesis during sporozoite-mediated malaria development reveals multiple response points
| العنوان: | Systematic tracking of altered haematopoiesis during sporozoite-mediated malaria development reveals multiple response points |
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| المؤلفون: | Vainieri, ML, Blagborough, AM, MacLean, AL, Haltalli, ML, Ruivo, N, Fletcher, HA, Stumpf, MP, Sinden, R, Celso, CL |
| المساهمون: | Haltalli, Myriam [0000-0002-0886-4466], Apollo - University of Cambridge Repository |
| المصدر: | Open Biology, Vol 6, Iss 6 (2016) Open Biology |
| بيانات النشر: | The Royal Society, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Research, plasmodium infection, Hematopoietic Stem Cells, Hematopoiesis, Malaria, Disease Models, Animal, Mice, lcsh:Biology (General), Sporozoites, population dynamics, Animals, lcsh:QH301-705.5, Research Article, haematopoietic stem cells, Cell Proliferation |
| الوصف: | Haematopoiesis is the complex developmental process that maintains the turnover of all blood cell lineages. It critically depends on the correct functioning of rare, quiescent haematopoietic stem cells (HSCs) and more numerous, HSC-derived, highly proliferative and differentiating haematopoietic progenitor cells (HPCs). Infection is known to affect HSCs, with severe and chronic inflammatory stimuli leading to stem cell pool depletion, while acute, non-lethal infections exert transient and even potentiating effects. Both whether this paradigm applies to all infections and whether the HSC response is the dominant driver of the changes observed during stressed haematopoiesis remain open questions. We use a mouse model of malaria, based on natural, sporozoite-driven Plasmodium berghei infection, as an experimental platform to gain a global view of haematopoietic perturbations during infection progression. We observe coordinated responses by the most primitive HSCs and multiple HPCs, some starting before blood parasitaemia is detected. We show that, despite highly variable inter-host responses, primitive HSCs become highly proliferative, but mathematical modelling suggests that this alone is not sufficient to significantly impact the whole haematopoietic cascade. We observe that the dramatic expansion of Sca-1(+) progenitors results from combined proliferation of direct HSC progeny and phenotypic changes in downstream populations. We observe that the simultaneous perturbation of HSC/HPC population dynamics is coupled with early signs of anaemia onset. Our data uncover a complex relationship between Plasmodium and its host's haematopoiesis and raise the question whether the variable responses observed may affect the outcome of the infection itself and its long-term consequences on the host. |
| وصف الملف: | application/pdf |
| تدمد: | 2046-2441 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::5ffd2198521e49b0fb27cd21e81778bd http://hdl.handle.net/10044/1/33612 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.pmid.dedup....5ffd2198521e49b0fb27cd21e81778bd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20462441 |
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