Beyond Uterine Natural Killer Cell Numbers in Unexplained Recurrent Pregnancy Loss: Combined Analysis of CD45, CD56, CD16, CD57, and CD138
| العنوان: | Beyond Uterine Natural Killer Cell Numbers in Unexplained Recurrent Pregnancy Loss: Combined Analysis of CD45, CD56, CD16, CD57, and CD138 |
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| المؤلفون: | Chiokadze, Maia, Bär, Christin, Pastuschek, Jana, Dons’koi, Boris V., Khazhylenko, Kseniia G., Schleußner, Ekkehard, Markert, Udo R., Favaro, Rodolfo R. |
| المصدر: | Diagnostics, Vol 10, Iss 650, p 650 (2020) Diagnostics Volume 10 Issue 9 |
| بيانات النشر: | MDPI AG, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | lcsh:R5-920, recurrent pregnancy loss, natural killer cells, hemic and immune systems, chemical and pharmacologic phenomena, Article, CD138, TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY, embryonic structures, CD56, human, CD45, endometrium, lcsh:Medicine (General), CD57, CD16 |
| الوصف: | Changes in the number and cytotoxic potential of uterine Natural Killer (uNK) cells have been associated with reduced fertility. To provide a better characterization of immunophenotypes in the endometrium of women with uRPL (unexplained recurrent pregnancy loss), we examined the applicability of a set of five immune cell markers. The concentration (cells/mm2) of CD45+ leukocytes, CD56+ uNK cells, and CD138+ plasma cells as well as of CD16+ and CD57+ cells, which indicate high cytotoxic uNK cells, were assessed by immunohistochemistry in endometrial biopsies from 61 uRPL patients and 10 controls. Control fertile endometria presented 90&ndash 300 CD56+ uNK cells/mm2. uRPL cases were classified in subgroups of low (uRPL-CD56low < 90 cells/mm2), normal (uRPL-CD56normal 90&ndash 300 cells/mm2), and high uNK cell counts (uRPL-CD56high > 300 cells/mm2). Some cases from the uRPL-CD56low and uRPL-CD56normal subgroups showed elevated proportions of cytotoxic CD16+ and CD57+ cells in relation to CD56+ cells. In the uRPL-CD56high subgroup, the CD57/CD56 ratio was reduced in most samples and the CD16/CD56 ratio was unaltered. Analysis of CD138 excluded the influence of chronic endometritis on these observations. Our results reinforce a link between uRPL and a dysfunctional endometrial environment associated with distinct immune cell profiles. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2075-4418 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::b9b0c42f6ed06e70e170fbf9ef2e64d5 https://www.mdpi.com/2075-4418/10/9/650 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.pmid.dedup....b9b0c42f6ed06e70e170fbf9ef2e64d5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20754418 |
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