Evaluation of a novel quantitative canine species-specific point-of-care assay for C-reactive protein
| العنوان: | Evaluation of a novel quantitative canine species-specific point-of-care assay for C-reactive protein |
|---|---|
| المؤلفون: | Sarah, Hindenberg, Melanie, Keßler, Sabine, Zielinsky, Judith, Langenstein, Andreas, Moritz, Natali, Bauer |
| المساهمون: | Department of Veterinary Clinical Sciences, Clinical Pathology and Clinical Pathophysiology |
| المصدر: | BMC Veterinary Research 14:99 doi: 10.1186/s12917-018-1415-2 BMC Veterinary Research, Vol 14, Iss 1, Pp 1-12 (2018) BMC Veterinary Research |
| بيانات النشر: | Justus-Liebig-Universität Gießen, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, Method validation, Total allowable error, canine, Acute phase protein, Canine, C-reactive protein, Dogs, Species Specificity, Bias, Heparin plasma, Point-of-care analyzer, Animals, ddc:630, Point-of-care assay, lcsh:Veterinary medicine, acute phase protein, Reproducibility of Results, method validation, Agriculture, point-of-care assay, point-of-care analyzer, Point-of-Care Testing, lcsh:SF600-1100, Female, Interference, Research Article |
| الوصف: | Background: Species-specific point-of-care tests (POCT) permit a rapid analysis of canine C-reactive protein (CRP), enabling veterinarians to include CRP in clinical decisions. Aim of the study was to evaluate a novel POCT for canine CRP (Point Stripâ TM Canine CRP Assay) run on a small in-house-analyzer (Point Reader TM V) using lithium heparin plasma and to compare assay performance to an already established canine CRP assay (Gentian Canine CRP Immunoassay) run on two different bench top analyzers serving as reference methods (ABX Pentra 400, AU 5800). Linearity was assessed by stepwise dilution of plasma samples with high CRP concentrations. Limit of quantification (LoQ) was determined by repeated measurements of samples with low CRP concentrations. Coefficient of variation (CV) at low (10-50 mg/l), moderate (50-100 mg/l), and high (100-200 mg/l) CRP concentrations was investigated as well as possible interferences. Method comparison study was performed using 45 samples of healthy and diseased dogs. Quality criteria were fulfilled if the total observed error (TEobs=2CV%+bias%) was below the minimal total allowable error of 44.4% (TE min). Additionally, a reference range (n =60 healthy dogs) was established. Results: Linearity was present at CRP concentrations of 10-132 mg/l (≙ 361 mg/l CRP with reference method) with a LoQ set at 10 mg/l. At moderate to high CRP concentrations, intra- and inter-assay CVs were< =8% and |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::c711950d7cb3ead307e7bbfa3bff2456 http://geb.uni-giessen.de/geb/volltexte/2019/14601/ |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.pmid.dedup....c711950d7cb3ead307e7bbfa3bff2456 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |