Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer
| العنوان: | Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer |
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| المؤلفون: | Kodahl, Annette R., Ehmsen, Sidse, Pallisgaard, Niels, Jylling, Anne M. B., Jensen, Jeanette D., Lænkholm, Anne‐Vibeke, Knoop, Ann S., Ditzel, Henrik J. |
| المصدر: | Molecular Oncology Molecular Oncology, Vol 12, Iss 6, Pp 925-935 (2018) |
| بيانات النشر: | John Wiley and Sons Inc., 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | liquid biopsy, Class I Phosphatidylinositol 3-Kinases, DNA Mutational Analysis, ddPCR, Breast Neoplasms, PIK3CA, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, lcsh:RC254-282, Circulating Tumor DNA, Treatment Outcome, Biomarkers, Tumor, Humans, Point Mutation, Female, metastatic breast cancer, Neoplasm Metastasis, neoplasms, Research Articles, Research Article |
| الوصف: | Liquid biopsies focusing on the analysis of cell‐free circulating tumor DNA (ctDNA) may have important clinical implications for personalized medicine, including early detection of cancer, therapeutic guidance, and monitoring of recurrence. Mutations in the oncogene, PIK3CA, are frequently observed in breast cancer and have been suggested as a predictive biomarker for PI3K‐selective inhibitor treatment. In this study, we analyzed the presence of PIK3CA mutations in formalin‐fixed, paraffin‐embedded, metastatic tissue and corresponding ctDNA from serum of patients with advanced breast cancer using a highly sensitive, optimized droplet digital PCR (ddPCR) assay. We found 83% of patients with PIK3CA mutation in the metastatic tumor tissue also had detectable PIK3CA mutations in serum ctDNA. Patients lacking the PIK3CA mutation in corresponding serum ctDNA all had nonvisceral metastatic disease. Four patients with detectable PIK3CA‐mutated ctDNA were followed with an additional serum sample during oncological treatment. In all cases, changes in PIK3CA ctDNA level correlated with treatment response. Our results showed high concordance between detection of PIK3CA mutations in tumor tissue and in corresponding serum ctDNA and suggest that serum samples from patients with advanced breast cancer and ddPCR may be used for PIK3CA mutation status assessment to complement imaging techniques as an early marker of treatment response. |
| اللغة: | English |
| تدمد: | 1878-0261 1574-7891 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::d4d75b752a2f1a2e30023d64df6436ec http://europepmc.org/articles/PMC5983134 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.pmid.dedup....d4d75b752a2f1a2e30023d64df6436ec |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18780261 15747891 |
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