Prevalence of ExoY Activity in Pseudomonas aeruginosa Reference Panel Strains and Impact on Cytotoxicity in Epithelial Cells
| العنوان: | Prevalence of ExoY Activity in Pseudomonas aeruginosa Reference Panel Strains and Impact on Cytotoxicity in Epithelial Cells |
|---|---|
| المؤلفون: | Silistre, Hazel, Raoux-Barbot, Dorothée, Mancinelli, Federica, Sangouard, Flora, Dupin, Alice, Belyy, Alexander, Deruelle, Vincent, Renault, Louis, Ladant, Daniel, Touqui, Lhousseine, Mechold, Undine |
| المساهمون: | Microbiologie structurale - Structural Microbiology (Microb. Struc. (UMR_3528 / U-Pasteur_5)), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Département de Biologie structurale et Chimie - Department of Structural Biology and Chemistry, Institut Pasteur [Paris], Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université de Paris (UP), Biochimie des Interactions Macromoléculaires / Biochemistry of Macromolecular Interactions, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Saint-Antoine (CR Saint-Antoine), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), ANR-18-CE44-0004,activExoY,Role et spécificités fonctionnelles des facteurs de virulence de type ExoY à activité Nucléotidyl Cyclase dépendante de l'actine dans les infections de bactéries à Gram-négatif(2018), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Université Paris Cité (UPCité), Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Gestionnaire, HAL Sorbonne Université 5, APPEL À PROJETS GÉNÉRIQUE 2018 - Role et spécificités fonctionnelles des facteurs de virulence de type ExoY à activité Nucléotidyl Cyclase dépendante de l'actine dans les infections de bactéries à Gram-négatif - - activExoY2018 - ANR-18-CE44-0004 - AAPG2018 - VALID |
| المصدر: | Frontiers in Microbiology Frontiers in Microbiology, Frontiers Media, 2021, 12, pp.666097. ⟨10.3389/fmicb.2021.666097⟩ Frontiers in Microbiology, 2021, 12, pp.666097. ⟨10.3389/fmicb.2021.666097⟩ |
| بيانات النشر: | Frontiers Media S.A., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | cystic fibrosis, cGMP, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ExoY, Pseudomonas aeruginosa, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, Microbiology, nucleotidyl cyclase, Original Research |
| الوصف: | International audience; ExoY is among the effectors that are injected by the type III secretion system (T3SS) of Pseudomonas aeruginosa into host cells. Inside eukaryotic cells, ExoY interacts with F-actin, which stimulates its potent nucleotidyl cyclase activity to produce cyclic nucleotide monophosphates (cNMPs). ExoY has broad substrate specificity with GTP as a preferential substrate in vitro. How ExoY contributes to the virulence of P. aeruginosa remains largely unknown. Here, we examined the prevalence of active ExoY among strains from the international P. aeruginosa reference panel, a collection of strains that includes environmental and clinical isolates, commonly used laboratory strains, and sequential clonal isolates from cystic fibrosis (CF) patients and thus represents the large diversity of this bacterial species. The ability to secrete active ExoY was determined by measuring the F-actin stimulated guanylate cyclase (GC) activity in bacterial culture supernatants. We found an overall ExoY activity prevalence of about 60% among the 40 examined strains with no significant difference between CF and non-CF isolates. In parallel, we used cellular infection models of human lung epithelial cells to compare the cytotoxic effects of isogenic reference strains expressing active ExoY or lacking the exoY gene. We found that P. aeruginosa strains lacking ExoY were in fact more cytotoxic to the epithelial cells than those secreting active ExoY. This suggests that under certain conditions, ExoY might partly alleviate the cytotoxic effects of other virulence factors of P. aeruginosa. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1664-302X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::f3f0f3657b785807f814e390ea9d631c http://europepmc.org/articles/PMC8524455 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.pmid.dedup....f3f0f3657b785807f814e390ea9d631c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1664302X |
|---|