Stable Isotope Probing-RNA Strategy to Study Plant/Fungus Interactions
العنوان: | Stable Isotope Probing-RNA Strategy to Study Plant/Fungus Interactions |
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المؤلفون: | Lê Van, Amandine, Duhamel, Marie, Quaiser, Achim, Vandenkoornhuyse, Philippe |
المساهمون: | Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Wageningen University and Research [Wageningen] (WUR) |
المصدر: | Methods in Molecular Biology Methods in Molecular Biology, 2023, 2605, pp.169-186. ⟨10.1007/978-1-0716-2871-3_9⟩ |
سنة النشر: | 2022 |
مصطلحات موضوعية: | Carbon thirteen, Fungal plant symbiont, RNA, qRT-PCR, Carbon transfer, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Stable-isotope probing (SIP) |
الوصف: | International audience; The use of stable-isotope probing (SIP) allows tracing specific labeled substrates into fungi leading to a better understanding of their role in biogeochemical cycles and their relationship with their environment. Stable isotope probing combined with ribosomal RNA molecule, conserved in the three kingdoms of life, and messenger RNA analysis permits the linkage of diversity and function. Here, we describe two methods designed to investigate the interactions between plants and their associated mycorrhizal compartment by tracing carbon flux from the host plant to its symbionts. |
تدمد: | 1940-6029 1064-3745 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::fde685e40a9fc8508d7e20ad772472ce https://pubmed.ncbi.nlm.nih.gov/36520394 |
رقم الأكسشن: | edsair.pmid.dedup....fde685e40a9fc8508d7e20ad772472ce |
قاعدة البيانات: | OpenAIRE |
تدمد: | 19406029 10643745 |
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