تقرير
Transcript length mediates developmental timing of gene expression across Drosophila
| العنوان: | Transcript length mediates developmental timing of gene expression across Drosophila |
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| المؤلفون: | Artieri, Carlo G., Fraser, Hunter B. |
| سنة النشر: | 2013 |
| المجموعة: | Quantitative Biology |
| مصطلحات موضوعية: | Quantitative Biology - Genomics, Quantitative Biology - Populations and Evolution |
| الوصف: | The time required to transcribe genes with long primary transcripts may limit their ability to be expressed in cells with short mitotic cycles, a phenomenon termed intron delay. As such short cycles are a hallmark of the earliest stages of insect development, we used Drosophila developmental timecourse expression data to test whether intron delay affects gene expression genome-wide, and to determine its consequences for the evolution of gene structure. We find that long zygotically expressed, but not maternally deposited, genes show substantial delay in expression relative to their shorter counterparts and that this delay persists over a substantial portion of the ~24 hours of embryogenesis. Patterns of RNA-seq coverage from the 5' and 3' ends of transcripts show that this delay is consistent with their inability to terminate transcription, but not with transcriptional initiation-based regulatory control. Highly expressed zygotic genes are subject to purifying selection to maintain compact transcribed regions, allowing conservation of embryonic expression patterns across the Drosophila phylogeny. We propose that intron delay is an underappreciated physical mechanism affecting both patterns of expression as well as gene structure of many genes across Drosophila. Comment: 31 pages, 5 figures, 1 table, 1 supplemental document, 5 supplemental figures, 3 supplemental tables |
| نوع الوثيقة: | Working Paper |
| URL الوصول: | http://arxiv.org/abs/1301.4516 |
| رقم الأكسشن: | edsarx.1301.4516 |
| قاعدة البيانات: | arXiv |
| الوصف غير متاح. |