تقرير
Evolution at two levels of gene expression in yeast
| العنوان: | Evolution at two levels of gene expression in yeast |
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| المؤلفون: | Artieri, Carlo G., Fraser, Hunter B. |
| سنة النشر: | 2013 |
| المجموعة: | Quantitative Biology |
| مصطلحات موضوعية: | Quantitative Biology - Genomics, Quantitative Biology - Populations and Evolution |
| الوصف: | Despite the greater functional importance of protein levels, our knowledge of gene expression evolution is based almost entirely on studies of mRNA levels. In contrast, our understanding of how translational regulation evolves has lagged far behind. Here we have applied ribosome profiling - which measures both global mRNA levels and their translation rates - to two species of Saccharomyces yeast and their interspecific hybrid in order to assess the relative contributions of changes in mRNA abundance and translation to regulatory evolution. We report that both cis and trans-acting regulatory divergence in translation are abundant, affecting at least 35% of genes. The majority of translational divergence acts to buffer changes in mRNA abundance, suggesting a widespread role for stabilizing selection acting across regulatory levels. Nevertheless, we observe evidence of lineage-specific selection acting on a number of yeast functional modules, including instances of reinforcing selection acting at both levels of regulation. Finally, we also uncover multiple instances of stop-codon readthrough that are conserved between species. Our analysis reveals the under-appreciated complexity of post-transcriptional regulatory divergence and indicates that partitioning the search for the locus of selection into the binary categories of 'coding' vs. 'regulatory' may overlook a significant source of selection, acting at multiple regulatory levels along the path from genotype to phenotype. Comment: 35 pages, 4 figures, 1 Supplemental Document |
| نوع الوثيقة: | Working Paper |
| URL الوصول: | http://arxiv.org/abs/1311.7140 |
| رقم الأكسشن: | edsarx.1311.7140 |
| قاعدة البيانات: | arXiv |
| الوصف غير متاح. |