تقرير
Salt-specific effects in lysozyme solutions
| العنوان: | Salt-specific effects in lysozyme solutions |
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| المؤلفون: | Janc, T., Kastelic, M., Boncina, M., Vlachy, V. |
| المصدر: | Condens. Matter Phys., 2016, vol. 19, No. 2, 23601 |
| سنة النشر: | 2016 |
| المجموعة: | Condensed Matter |
| مصطلحات موضوعية: | Condensed Matter - Soft Condensed Matter |
| الوصف: | The effects of additions of low-molecular-mass salts on the properties of aqueous lysozyme solutions are examined by using the cloud-point temperature, $T_{cloud}$, measurements. Mixtures of protein, buffer, and simple salt in water are studied at pH=6.8 (phosphate buffer) and pH=4.6 (acetate buffer). We show that an addition of buffer in the amount above $I_{buffer} = 0.6$ mol dm$^{-3}$ does not affect the $T_{cloud}$ values. However, by replacing a certain amount of the buffer electrolyte by another salt, keeping the total ionic strength constant, we can significantly change the cloud-point temperature. All the salts de-stabilize the solution and the magnitude of the effect depends on the nature of the salt. Experimental results are analyzed within the framework of the one-component model, which treats the protein-protein interaction as highly directional and of short-range. We use this approach to predict the second virial coefficients, and liquid-liquid phase diagrams under conditions, where $T_{cloud}$ is determined experimentally. Comment: 12 pages, 7 figures |
| نوع الوثيقة: | Working Paper |
| DOI: | 10.5488/CMP.19.23601 |
| URL الوصول: | http://arxiv.org/abs/1603.07114 |
| رقم الأكسشن: | edsarx.1603.07114 |
| قاعدة البيانات: | arXiv |
| DOI: | 10.5488/CMP.19.23601 |
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