An integrated single- and two-photon non-diffracting light-sheet microscope

التفاصيل البيبلوغرافية
العنوان: An integrated single- and two-photon non-diffracting light-sheet microscope
المؤلفون: Lau, Sze Cheung, Chiu, Hoi Chun, Zhao, Luwei, Zhao, Teng, Loy, M. M. T., Du, Shengwang
المصدر: Rev. Sci. Instrum. 89, 043701 (2018)
سنة النشر: 2017
المجموعة: Physics (Other)
مصطلحات موضوعية: Physics - Instrumentation and Detectors, Physics - Biological Physics, Physics - Optics
الوصف: We describe the apparatus of a fluorescence optical microscope with both single-photon and two-photon non-diffracting light sheets excitation for large volume imaging. With special design to accommodate two different wavelength ranges (visible: 400-700 nm, and near infrared: 800-1200 nm), we combine the line-Bessel sheet (LBS, for single-photon excitation) and the scanning Bessel beam (SBB, for two-photon excitation) light sheet together in a single microscope setup. For a transparent thin sample where the scattering can be ignored, the LBS single-photon excitation is the optimal imaging solution. When the light scattering becomes significant for a deep-cell or deep-tissue imaging, we use SBB light-sheet two-photon excitation with a longer wavelength. We achieved nearly identical lateral/axial resolution of about 350/270 nm for both imagings. This integrated light-sheet microscope may have a wide application for live-cell and live-tissue three-dimensional high-speed imaging.
Comment: 4 pages, 5 figures
نوع الوثيقة: Working Paper
DOI: 10.1063/1.5020154
URL الوصول: http://arxiv.org/abs/1712.02521
رقم الأكسشن: edsarx.1712.02521
قاعدة البيانات: arXiv