Effective bowel motion reduction in mouse abdominal MRI using hyoscine butylbromide

التفاصيل البيبلوغرافية
العنوان: Effective bowel motion reduction in mouse abdominal MRI using hyoscine butylbromide
المؤلفون: Bilreiro, Carlos, Fernandes, Francisca F., Andrade, Luísa, Chavarrías, Cristina, Simões, Rui V., Matos, Celso, Shemesh, Noam
المصدر: Magn Reson Med, 2021
سنة النشر: 2020
المجموعة: Physics (Other)
مصطلحات موضوعية: Physics - Medical Physics
الوصف: Purpose: Bowel motion is a significant source of artifacts in mouse abdominal MRI. Fasting and administration of hyoscine butylbromide (BUSC) have been proposed for bowel motion reduction, but with inconsistent results and limited efficacy assessments. Here, we evaluate these regimes for mouse abdominal MRI at high field. Methods: Thirty-two adult C57BL/6J mice were imaged on a 9.4T scanner with a FLASH sequence, acquired over 90 minutes with ~19s temporal resolution. During MRI acquisition, eight mice were injected with a low-dose and eight mice with a high-dose bolus of BUSC (0.5 and 5 mg/kg, respectively). Eight mice were food deprived for 4.5-6.5h before MRI and another group of 8 mice was injected with saline during MRI acquisition. Two expert readers reviewed the images and classified bowel motion, and quantitative voxel-wise analyses were performed for identification of moving regions. After defining the most effective protocol, high-resolution T2-weighted and diffusion-weighted images were acquired from four mice. Results: High-dose BUSC was the most effective protocol for bowel motion reduction, for up to 45 minutes. Fasting and saline protocols were not effective in suppressing bowel motion. High-resolution abdominal MRI clearly demonstrated improved image quality and ADC quantification with the high-dose BUSC protocol. Conclusion: Our data show that BUSC administration is advantageous for abdominal MRI in the mouse. Specifically, it endows significant bowel motion reduction, with relatively short onset timings after injection (~8.5 minutes) and relatively long duration of the effect (~45 minutes). These features improve the quality of high-resolution images of the mouse abdomen.
Comment: Carlos Bilreiro and Francisca F. Fernandes contributed equally to this work
نوع الوثيقة: Working Paper
DOI: 10.1002/mrm.28824
URL الوصول: http://arxiv.org/abs/2007.04282
رقم الأكسشن: edsarx.2007.04282
قاعدة البيانات: arXiv