تقرير
Ligation of random oligomers leads to emergence of autocatalytic sequence network
| العنوان: | Ligation of random oligomers leads to emergence of autocatalytic sequence network |
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| المؤلفون: | Kudella, Patrick W., Tkachenko, Alexei V., Maslov, Sergei, Braun, Dieter |
| سنة النشر: | 2020 |
| المجموعة: | Physics (Other) |
| مصطلحات موضوعية: | Physics - Biological Physics |
| الوصف: | The emergence of longer information-carrying and functional nucleotide polymers from random short strands was a major stepping stone at the dawn of life. But the formation of those polymers under temperature oscillation required some form of selection. A plausible mechanism is template-based ligation where theoretical work already suggested a reduction in information entropy. Here, we show how nontrivial sequence patterns emerge in a system of random 12mer DNA sequences subject to enzyme-based templated ligation reaction and temperature cycling. The strands acted both as a template and substrates of the reaction and thereby formed longer oligomers. The selection for templating sequences leads to the development of a multiscale ligation landscape. A position-dependent sequence pattern emerged with a segregation into mutually complementary pools of A-rich and T-rich sequences. Even without selection for function, the base pairing of DNA with ligation showed a dynamics resembling Darwinian evolution. Comment: 11 pages, 5 figures, SI attached |
| نوع الوثيقة: | Working Paper |
| URL الوصول: | http://arxiv.org/abs/2008.07823 |
| رقم الأكسشن: | edsarx.2008.07823 |
| قاعدة البيانات: | arXiv |
| الوصف غير متاح. |