تقرير
Probing cage relaxation in concentrated protein solutions by XPCS
| العنوان: | Probing cage relaxation in concentrated protein solutions by XPCS |
|---|---|
| المؤلفون: | Chushkin, Yuriy, Gulotta, Alessandro, Roosen-Runge, Felix, Pal, Antara, Stradner, Anna, Schurtenberger, Peter |
| سنة النشر: | 2022 |
| المجموعة: | Condensed Matter Physics (Other) |
| مصطلحات موضوعية: | Condensed Matter - Soft Condensed Matter, Physics - Biological Physics |
| الوصف: | Diffusion of proteins on length scales of their own diameter in highly concentrated solutions is essential for understanding the cellular machinery of living cells, but its experimental characterization remains a challenge. While X-ray photon correlation spectroscopy (XPCS) is currently the only technique that in principle allows for a measurement of long-time collective diffusion on these length scales for such systems, its application to protein solutions is seriously hampered by radiation damage caused by the highly intense X-ray beams required for such experiments. Here we apply an experimental design and an analysis strategy that allow us to successfully use XPCS experiments in order to measure collective long-time cage relaxation in highly crowded solutions of the eye lens protein {\alpha}-crystallin close to and beyond dynamical arrest. We also address the problem of radiation-induced damage in such experiments. We demonstrate that radiation effects depend both on the total dose as well as the dose rate of the absorbed radiation, and discuss possible processes and mechanism responsible for the observed radiation effects as well as their consequences for future applications of XPCS in biological systems. |
| نوع الوثيقة: | Working Paper |
| URL الوصول: | http://arxiv.org/abs/2203.12695 |
| رقم الأكسشن: | edsarx.2203.12695 |
| قاعدة البيانات: | arXiv |
| الوصف غير متاح. |