تقرير
Force-Dependent Folding Kinetics of Single Molecules with Multiple Intermediates and Pathways
| العنوان: | Force-Dependent Folding Kinetics of Single Molecules with Multiple Intermediates and Pathways |
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| المؤلفون: | Rico-Pasto, Marc, Alemany, Anna, Ritort, Felix |
| المصدر: | The Journal of Physical Chemistry Letters 2022 13 (4), 1025-1032 |
| سنة النشر: | 2022 |
| المجموعة: | Condensed Matter |
| مصطلحات موضوعية: | Condensed Matter - Soft Condensed Matter, Condensed Matter - Statistical Mechanics |
| الوصف: | Most single-molecule studies derive the kinetic rates of native, intermediate, and unfolded states from equilibrium hopping experiments. Here, we apply Kramers kinetic diffusive model to derive the force-dependent kinetic rates of intermediate states from non-equilibrium pulling experiments. From the kinetic rates, we also extract the force-dependent kinetic barriers and the equilibrium folding energies. We apply our method to DNA hairpins with multiple folding pathways and intermediates. The experimental results agree with theoretical predictions. Furthermore, the proposed non-equilibrium single-molecule approach permits us to characterize kinetic and thermodynamic properties of native, unfolded, and intermediate states that cannot be derived from equilibrium hopping experiments. Comment: 8 pages, 5 figures |
| نوع الوثيقة: | Working Paper |
| DOI: | 10.1021/acs.jpclett.1c03521 |
| URL الوصول: | http://arxiv.org/abs/2204.05705 |
| رقم الأكسشن: | edsarx.2204.05705 |
| قاعدة البيانات: | arXiv |
| DOI: | 10.1021/acs.jpclett.1c03521 |
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