Based on the practical process of innovation and entrepreneurship education for college students in the author's university, this study analyzes and deconstructs the key concepts of AI knowledge-based crowdsourcing on the basis of literature research, and analyzes the objective fitting needs of combining AI knowledge-based crowdsourcing with college students' innovation and entrepreneurship education practice through a survey and research of a random sample of college students, and verifies that college students' knowledge and application of AI knowledge-based crowdsourcing in the learning and practice of innovation and entrepreneurship The study also verifies the awareness and application of AI knowledge-based crowdsourcing knowledge by university students in the learning and practice of innovation and entrepreneurship.