تقرير
Raman Spectroscopy Reveals Photobiomodulation-Induced {\alpha}-Helix to {\beta}-Sheet Transition in Tubulins: Potential Implications for Alzheimer's and Other Neurodegenerative Diseases
| العنوان: | Raman Spectroscopy Reveals Photobiomodulation-Induced {\alpha}-Helix to {\beta}-Sheet Transition in Tubulins: Potential Implications for Alzheimer's and Other Neurodegenerative Diseases |
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| المؤلفون: | Di Gregorio, Elisabetta, Staelens, Michael, Hosseinkhah, Nazanin, Karimpoor, Mahroo, Liburd, Janine, Lim, Lew, Shankar, Karthik, Tuszynski, Jack A. |
| سنة النشر: | 2023 |
| المجموعة: | Quantitative Biology |
| مصطلحات موضوعية: | Quantitative Biology - Biomolecules, Quantitative Biology - Quantitative Methods |
| الوصف: | In this study, we employed a Raman spectroscopic analysis of the amide I band of polymerized samples of tubulin exposed to pulsed low-intensity NIR radiation (810 nm, 10 Hz, 22.5 J/cm$^{2}$ dose). Peaks in the Raman fingerprint region (300$\unicode{x2013}$1900 cm$^{-1}$), in particular, in the amide I band (1600$\unicode{x2013}$1700 cm$^{-1}$), can be used to quantify the percentage of protein secondary structures. Under this band, hidden signals of $\mathrm{C}$=$\mathrm{O}$ stretching, belonging to different structures, are superimposed$\unicode{x2014}$producing a complex signal as a result. An accurate decomposition of the amide I band is therefore required for the reliable analysis of the conformation of proteins, which we achieved through a straightforward method employing a Voigt profile. This approach was validated through secondary structure analyses of unexposed control samples, for which comparisons with other values available in the literature could be conducted. Subsequently, using this validated method, we present novel findings of statistically significant alterations in the secondary structures of NIR-exposed tubulin, characterized by a notable decrease in alpha-helix content and a concurrent increase in beta-sheets compared to the control samples. The alpha-helix to beta-sheet transition suggests that PBM reduces microtubule stability and introduces dynamism to allow for the remodeling and, consequently, refreshing of microtubule structures. This newly discovered mechanism could have implications for reducing the risks associated with brain aging, including neurodegenerative diseases like Alzheimer's disease. Comment: 15 pages, 3 figures |
| نوع الوثيقة: | Working Paper |
| DOI: | 10.3390/nano14131093 |
| URL الوصول: | http://arxiv.org/abs/2311.04165 |
| رقم الأكسشن: | edsarx.2311.04165 |
| قاعدة البيانات: | arXiv |
| DOI: | 10.3390/nano14131093 |
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