تقرير
Quantitative phase microscopies: accuracy comparison
| العنوان: | Quantitative phase microscopies: accuracy comparison |
|---|---|
| المؤلفون: | Chaumet, Patrick C., Bon, Pierre, Maire, Guillaume, Sentenac, Anne, Baffou, Guillaume |
| سنة النشر: | 2024 |
| المجموعة: | Physics (Other) |
| مصطلحات موضوعية: | Physics - Optics |
| الوصف: | This article presents a thorough comparison of themain QPM techniques, focusing on their accuracy in terms of measurement precision and trueness. We focus on 8 techniques, namely digital holographic microscopy (DHM), cross-grating wavefront microscopy (CGM), which is based on QLSI (quadriwave lateral shearing interferometry), diffraction phase microscopy (DPM), differential phase-contrast (DPC) microscopy, phase-shifting interferometry (PSI) imaging, Fourier phase microscopy (FPM), spatial light interference microscopy (SLIM), and transport-of-intensity equation (TIE) imaging. For this purpose, we used a home-made numerical toolbox based on discrete dipole approximation (IF-DDA). This toolbox is designed to compute the electromagnetic field at the sample plane of a microscope, irrespective of the object's complexity or the illumination conditions. We upgraded this toolbox to enable it to model any type of QPM, and to take into account shot noise. In a nutshell, the results show that DHM and PSI are inherently free from artefacts and rather suffer from coherent noise; In CGM, DPC, DPM and TIE, there is a trade off between precision and trueness, which can be balanced by varying one experimental parameter; FPM and SLIM suffer from inherent artefacts that cannot be discarded experimentally in most cases, making the techniques not quantitative especially for large objects covering a large part of the field of view, such as eukaryotic cells. Comment: 34 pages, 16 figures |
| نوع الوثيقة: | Working Paper |
| URL الوصول: | http://arxiv.org/abs/2403.11930 |
| رقم الأكسشن: | edsarx.2403.11930 |
| قاعدة البيانات: | arXiv |
| الوصف غير متاح. |