دورية أكاديمية
A method for the generation of pseudovirus particles bearing SARS coronavirus spike protein in high yields
| العنوان: | A method for the generation of pseudovirus particles bearing SARS coronavirus spike protein in high yields |
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| المؤلفون: | Yoichiro Fujioka, Sayaka Kashiwagi, Aiko Yoshida, Aya O. Satoh, Mari Fujioka, Maho Amano, Yohei Yamauchi, Yusuke Ohba |
| المصدر: | Cell Structure and Function, Vol 47, Iss 1, Pp 43-53 (2022) |
| بيانات النشر: | Japan Society for Cell Biology, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | severe acute respiratory syndrome coronavirus (sars-cov), sars-cov-2, pseudovirus, vesicular stomatitis virus (vsv), spike protein, Science, Biology (General), QH301-705.5 |
| الوصف: | The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has threatened human health and the global economy. Development of additional vaccines and therapeutics is urgently required, but such development with live virus must be conducted with biosafety level 3 confinement. Pseudotyped viruses have been widely adopted for studies of virus entry and pharmaceutical development to overcome this restriction. Here we describe a modified protocol to generate vesicular stomatitis virus (VSV) pseudotyped with SARS-CoV or SARS-CoV-2 spike protein in high yield. We found that a large proportion of pseudovirions produced with the conventional transient expression system lacked coronavirus spike protein at their surface as a result of inhibition of parental VSV infection by overexpression of this protein. Establishment of stable cell lines with an optimal expression level of coronavirus spike protein allowed the efficient production of progeny pseudoviruses decorated with spike protein. This improved VSV pseudovirus production method should facilitate studies of coronavirus entry and development of antiviral agents. Key words: severe acute respiratory syndrome coronavirus (SARS-CoV), SARS-CoV-2, pseudovirus, vesicular stomatitis virus (VSV), spike protein |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0386-7196 1347-3700 |
| Relation: | https://www.jstage.jst.go.jp/article/csf/47/1/47_21047/_html/-char/en; https://doaj.org/toc/0386-7196; https://doaj.org/toc/1347-3700 |
| DOI: | 10.1247/csf.21047 |
| URL الوصول: | https://doaj.org/article/00454e2c59ca410f89423e9c2c8c62fe |
| رقم الأكسشن: | edsdoj.00454e2c59ca410f89423e9c2c8c62fe |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 03867196 13473700 |
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| DOI: | 10.1247/csf.21047 |