دورية أكاديمية
An efficient screening system of disease-resistant genes from wild apple, Malus sieversii in response to Valsa mali pathogenic fungus
| العنوان: | An efficient screening system of disease-resistant genes from wild apple, Malus sieversii in response to Valsa mali pathogenic fungus |
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| المؤلفون: | Xuejing Wen, Jiangxue Yuan, Tohir A. Bozorov, Abdul Waheed, Gulnaz Kahar, Yakupjan Haxim, Xiaojie Liu, Lili Huang, Daoyuan Zhang |
| المصدر: | Plant Methods, Vol 19, Iss 1, Pp 1-16 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Plant culture LCC:Biology (General) |
| مصطلحات موضوعية: | Malus sieversii, Valsa mali, Disease resistant genes, Transiently transformation, Immune regulatory network, Plant culture, SB1-1110, Biology (General), QH301-705.5 |
| الوصف: | Abstract For molecular breeding of future apples, wild apple (Malus sieversii), the primary progenitor of domesticated apples, provides abundant genetic diversity and disease-resistance traits. Valsa canker (caused by the fungal pathogen Valsa mali) poses a major threat to wild apple population as well as to cultivated apple production in China. In the present study, we developed an efficient system for screening disease-resistant genes of M. sieversii in response to V. mali. An optimal agrobacterium-mediated transient transformation of M. sieversii was first used to manipulate in situ the expression of candidate genes. After that, the pathogen V. mali was inoculated on transformed leaves and stems, and 3 additional methods for slower disease courses were developed for V. mali inoculation. To identify the resistant genes, a series of experiments were performed including morphological (incidence, lesion area/length, fungal biomass), physiological (H2O2 content, malondialdehyde content), and molecular (Real-time quantitative Polymerase Chain Reaction) approaches. Using the optimized system, we identified two transcription factors with high resistance to V. mali, MsbHLH41 and MsEIL3. Furthermore, 35 and 45 downstream genes of MsbHLH41 and MsEIL3 were identified by screening the V. mali response gene database in M. sieversii, respectively. Overall, these results indicate that the disease-resistant gene screening system has a wide range of applications for identifying resistant genes and exploring their immune regulatory networks. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1746-4811 |
| Relation: | https://doaj.org/toc/1746-4811 |
| DOI: | 10.1186/s13007-023-01115-w |
| URL الوصول: | https://doaj.org/article/c0058dab3b73490a8e56e95a4d038103 |
| رقم الأكسشن: | edsdoj.0058dab3b73490a8e56e95a4d038103 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 17464811 |
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| DOI: | 10.1186/s13007-023-01115-w |