دورية أكاديمية
In vitro propagation of Liparis nervosa (Thunb.) Lindl., an endangered medicinal orchid
| العنوان: | In vitro propagation of Liparis nervosa (Thunb.) Lindl., an endangered medicinal orchid |
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| المؤلفون: | Yan Ren, Jin-Rong Gao, Shou-Meng Cai, Hong-Mei Yan, Heng-Yu Huang |
| المصدر: | Acta Scientiarum Polonorum: Hortorum Cultus, Vol 22, Iss 5 (2023) |
| بيانات النشر: | University of Life Sciences in Lublin - Publishing House, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biochemistry LCC:Plant culture LCC:Science |
| مصطلحات موضوعية: | in vitro regeneration, proliferation method, basal stem cluster bud, tiller bud, protocorm-like body, Liparis nervosa (Thunb.) Lindl., Biochemistry, QD415-436, Plant culture, SB1-1110, Science |
| الوصف: | In vitro regeneration was studied to protect the rare Chinese medicinal orchid Liparis nervosa (Thunb.) Lindl. The mixtures of protocorm and seeding and the stem tip were used as explants. The results revealed that the best essential medium for L. nervosa growth was 1/3 MS medium with 25 g · L–1 sucrose, 50 g · L–1 banana puree, 40 g · L–1 mashed potato, and 1.0 g · L–1 AC (MS1); MS1 medium with 0.5 mg · L–1 BA, 0.05 mg · L–1 2,4-D, and 1.5 mg · L–1 NAA was optimal for proliferation. When stem tips were cultured in a proliferation medium, four types of proliferation occurred: basal stem cluster bud (occurring at the basal node), tiller bud (occurring at the root), protocorm-like body (occurring at the plant’s base incision), and high-position bud (occurring on plant stem nodes other than the basal nodes). Four methods produced 10.12 proliferation coefficients. In the MS1 medium with 0.5 mg · L−1 NAA, the plantlets rooted 100%, and the rooted plantlets survived 100% after domestication and transplantation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1644-0692 2545-1405 |
| Relation: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/5023; https://doaj.org/toc/1644-0692; https://doaj.org/toc/2545-1405 |
| DOI: | 10.24326/asphc.2023.5023 |
| URL الوصول: | https://doaj.org/article/00bf3650cfea486ebd256617067aaaeb |
| رقم الأكسشن: | edsdoj.00bf3650cfea486ebd256617067aaaeb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16440692 25451405 |
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| DOI: | 10.24326/asphc.2023.5023 |