دورية أكاديمية
أعرض في EDS

X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature

التفاصيل البيبلوغرافية
العنوان: X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature
المؤلفون: Christopher D. M. Hutchison, Violeta Cordon-Preciado, Rhodri M. L. Morgan, Takanori Nakane, Josie Ferreira, Gabriel Dorlhiac, Alvaro Sanchez-Gonzalez, Allan S. Johnson, Ann Fitzpatrick, Clyde Fare, Jon P. Marangos, Chun Hong Yoon, Mark S. Hunter, Daniel P. DePonte, Sébastien Boutet, Shigeki Owada, Rie Tanaka, Kensuke Tono, So Iwata, Jasper J. van Thor
المصدر: International Journal of Molecular Sciences, Vol 18, Iss 9, p 1918 (2017)
بيانات النشر: MDPI AG, 2017.
سنة النشر: 2017
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: XFEL, SFX, rsFP, Skylan-NS, SACLA, LCLS, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: The photochromic fluorescent protein Skylan-NS (Nonlinear Structured illumination variant mEos3.1H62L) is a reversibly photoswitchable fluorescent protein which has an unilluminated/ground state with an anionic and cis chromophore conformation and high fluorescence quantum yield. Photo-conversion with illumination at 515 nm generates a meta-stable intermediate with neutral trans-chromophore structure that has a 4 h lifetime. We present X-ray crystal structures of the cis (on) state at 1.9 Angstrom resolution and the trans (off) state at a limiting resolution of 1.55 Angstrom from serial femtosecond crystallography experiments conducted at SPring-8 Angstrom Compact Free Electron Laser (SACLA) at 7.0 keV and 10.5 keV, and at Linac Coherent Light Source (LCLS) at 9.5 keV. We present a comparison of the data reduction and structure determination statistics for the two facilities which differ in flux, beam characteristics and detector technologies. Furthermore, a comparison of droplet on demand, grease injection and Gas Dynamic Virtual Nozzle (GDVN) injection shows no significant differences in limiting resolution. The photoconversion of the on- to the off-state includes both internal and surface exposed protein structural changes, occurring in regions that lack crystal contacts in the orthorhombic crystal form.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: https://www.mdpi.com/1422-0067/18/9/1918; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms18091918
URL الوصول: https://doaj.org/article/00f82ad034f34aaf80ff9d7707509803
رقم الأكسشن: edsdoj.00f82ad034f34aaf80ff9d7707509803
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms18091918