دورية أكاديمية
Archaeal DNA Polymerase-B as a DNA Template Guardian: Links between Polymerases and Base/Alternative Excision Repair Enzymes in Handling the Deaminated Bases Uracil and Hypoxanthine
| العنوان: | Archaeal DNA Polymerase-B as a DNA Template Guardian: Links between Polymerases and Base/Alternative Excision Repair Enzymes in Handling the Deaminated Bases Uracil and Hypoxanthine |
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| المؤلفون: | Javier Abellón-Ruiz, Sonoko Ishino, Yoshizumi Ishino, Bernard A. Connolly |
| المصدر: | Archaea, Vol 2016 (2016) |
| بيانات النشر: | Hindawi Limited, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | Microbiology, QR1-502 |
| الوصف: | In Archaea repair of uracil and hypoxanthine, which arise by deamination of cytosine and adenine, respectively, is initiated by three enzymes: Uracil-DNA-glycosylase (UDG, which recognises uracil); Endonuclease V (EndoV, which recognises hypoxanthine); and Endonuclease Q (EndoQ), (which recognises both uracil and hypoxanthine). Two archaeal DNA polymerases, Pol-B and Pol-D, are inhibited by deaminated bases in template strands, a feature unique to this domain. Thus the three repair enzymes and the two polymerases show overlapping specificity for uracil and hypoxanthine. Here it is demonstrated that binding of Pol-D to primer-templates containing deaminated bases inhibits the activity of UDG, EndoV, and EndoQ. Similarly Pol-B almost completely turns off EndoQ, extending earlier work that demonstrated that Pol-B reduces catalysis by UDG and EndoV. Pol-B was observed to be a more potent inhibitor of the enzymes compared to Pol-D. Although Pol-D is directly inhibited by template strand uracil, the presence of Pol-B further suppresses any residual activity of Pol-D, to near-zero levels. The results are compatible with Pol-D acting as the replicative polymerase and Pol-B functioning primarily as a guardian preventing deaminated base-induced DNA mutations. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1472-3646 1472-3654 |
| Relation: | https://doaj.org/toc/1472-3646; https://doaj.org/toc/1472-3654 |
| DOI: | 10.1155/2016/1510938 |
| URL الوصول: | https://doaj.org/article/0199decf62f24f5d908ff7223c84e345 |
| رقم الأكسشن: | edsdoj.0199decf62f24f5d908ff7223c84e345 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14723646 14723654 |
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| DOI: | 10.1155/2016/1510938 |