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Mechanistic insights into validoxylamine A 7'-phosphate synthesis by VldE using the structure of the entire product complex.

التفاصيل البيبلوغرافية
العنوان: Mechanistic insights into validoxylamine A 7'-phosphate synthesis by VldE using the structure of the entire product complex.
المؤلفون: Michael C Cavalier, Young-Sun Yim, Shumpei Asamizu, David Neau, Khaled H Almabruk, Taifo Mahmud, Yong-Hwan Lee
المصدر: PLoS ONE, Vol 7, Iss 9, p e44934 (2012)
بيانات النشر: Public Library of Science (PLoS), 2012.
سنة النشر: 2012
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: The pseudo-glycosyltransferase VldE catalyzes non-glycosidic C-N coupling between an unsaturated cyclitol and a saturated aminocyclitol with the conservation of the stereochemical configuration of the substrates to form validoxylamine A 7'-phosphate, the biosynthetic precursor of the antibiotic validamycin A. To study the molecular basis of its mechanism, the three-dimensional structures of VldE from Streptomyces hygroscopicus subsp. limoneus was determined in apo form, in complex with GDP, in complex with GDP and validoxylamine A 7'-phosphate, and in complex with GDP and trehalose. The structure of VldE with the catalytic site in both an "open" and "closed" conformation is also described. With these structures, the preferred binding of the guanine moiety by VldE, rather than the uracil moiety as seen in OtsA could be explained. The elucidation of the VldE structure in complex with the entirety of its products provides insight into the internal return mechanism by which catalysis occurs with a net retention of the stereochemical configuration of the donated cyclitol.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3441724?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0044934
URL الوصول: https://doaj.org/article/022bc0efb7af4be0bd5020bf7b3b07c1
رقم الأكسشن: edsdoj.022bc0efb7af4be0bd5020bf7b3b07c1
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0044934