دورية أكاديمية
Cut&tag: a powerful epigenetic tool for chromatin profiling
| العنوان: | Cut&tag: a powerful epigenetic tool for chromatin profiling |
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| المؤلفون: | Zhijun Fu, Sanjie Jiang, Yiwen Sun, Shanqiao Zheng, Liang Zong, Peipei Li |
| المصدر: | Epigenetics, Vol 19, Iss 1 (2024) |
| بيانات النشر: | Taylor & Francis Group, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Genetics |
| مصطلحات موضوعية: | CUT&Tag, chromatin profiling, epigenetics, Tn5 tagmentation, single-cell CUT&Tag, Genetics, QH426-470 |
| الوصف: | ABSTRACTAnalysis of transcription factors and chromatin modifications at the genome-wide level provides insights into gene regulatory processes, such as transcription, cell differentiation and cellular response. Chromatin immunoprecipitation is the most popular and powerful approach for mapping chromatin, and other enzyme-tethering techniques have recently become available for living cells. Among these, Cleavage Under Targets and Tagmentation (CUT&Tag) is a relatively novel chromatin profiling method that has rapidly gained popularity in the field of epigenetics since 2019. It has also been widely adapted to map chromatin modifications and TFs in different species, illustrating the association of these chromatin epitopes with various physiological and pathological processes. Scalable single-cell CUT&Tag can be combined with distinct platforms to distinguish cellular identity, epigenetic features and even spatial chromatin profiling. In addition, CUT&Tag has been developed as a strategy for joint profiling of the epigenome, transcriptome or proteome on the same sample. In this review, we will mainly consolidate the applications of CUT&Tag and its derivatives on different platforms, give a detailed explanation of the pros and cons of this technique as well as the potential development trends and applications in the future. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 15592294 1559-2308 1559-2294 |
| Relation: | https://doaj.org/toc/1559-2294; https://doaj.org/toc/1559-2308 |
| DOI: | 10.1080/15592294.2023.2293411 |
| URL الوصول: | https://doaj.org/article/02b2c60adaab4d7bac17659c1069b253 |
| رقم الأكسشن: | edsdoj.02b2c60adaab4d7bac17659c1069b253 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 15592294 15592308 |
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| DOI: | 10.1080/15592294.2023.2293411 |