دورية أكاديمية
Deciphering the regulation of P2X4 receptor channel gating by ivermectin using Markov models.
العنوان: | Deciphering the regulation of P2X4 receptor channel gating by ivermectin using Markov models. |
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المؤلفون: | Laurent Mackay, Hana Zemkova, Stanko S Stojilkovic, Arthur Sherman, Anmar Khadra |
المصدر: | PLoS Computational Biology, Vol 13, Iss 7, p e1005643 (2017) |
بيانات النشر: | Public Library of Science (PLoS), 2017. |
سنة النشر: | 2017 |
المجموعة: | LCC:Biology (General) |
مصطلحات موضوعية: | Biology (General), QH301-705.5 |
الوصف: | The P2X4 receptor (P2X4R) is a member of a family of purinergic channels activated by extracellular ATP through three orthosteric binding sites and allosterically regulated by ivermectin (IVM), a broad-spectrum antiparasitic agent. Treatment with IVM increases the efficacy of ATP to activate P2X4R, slows both receptor desensitization during sustained ATP application and receptor deactivation after ATP washout, and makes the receptor pore permeable to NMDG+, a large organic cation. Previously, we developed a Markov model based on the presence of one IVM binding site, which described some effects of IVM on rat P2X4R. Here we present two novel models, both with three IVM binding sites. The simpler one-layer model can reproduce many of the observed time series of evoked currents, but does not capture well the short time scales of activation, desensitization, and deactivation. A more complex two-layer model can reproduce the transient changes in desensitization observed upon IVM application, the significant increase in ATP-induced current amplitudes at low IVM concentrations, and the modest increase in the unitary conductance. In addition, the two-layer model suggests that this receptor can exist in a deeply inactivated state, not responsive to ATP, and that its desensitization rate can be altered by each of the three IVM binding sites. In summary, this study provides a detailed analysis of P2X4R kinetics and elucidates the orthosteric and allosteric mechanisms regulating its channel gating. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1553-734X 1553-7358 |
Relation: | http://europepmc.org/articles/PMC5533465?pdf=render; https://doaj.org/toc/1553-734X; https://doaj.org/toc/1553-7358 |
DOI: | 10.1371/journal.pcbi.1005643 |
URL الوصول: | https://doaj.org/article/0311971a97b240bb8329befcaa5cce3b |
رقم الأكسشن: | edsdoj.0311971a97b240bb8329befcaa5cce3b |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 1553734X 15537358 |
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DOI: | 10.1371/journal.pcbi.1005643 |