دورية أكاديمية
IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein
| العنوان: | IgM and IgG Immunoreactivity of SARS-CoV-2 Recombinant M Protein |
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| المؤلفون: | Zorana Lopandić, Isidora Protić-Rosić, Aleksandra Todorović, Sofija Glamočlija, Marija Gnjatović, Danica Ćujic, Marija Gavrović-Jankulović |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 9, p 4951 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | SARS-CoV-2 membrane protein, COVID-19, IgM reactivity, IgG reactivity, antigenicity, ELISA, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Diagnostic evaluation of specific antibodies against the SARS-CoV-2 virus is mainly based on spike (S) and nucleocapsid (N) proteins. Despite the critical functions in virus infection and contribution to the pattern of immunodominance in COVID-19, exploitation of the most abundant membrane (M) protein in the SARS-CoV-2 serology tests is minimal. This study investigated the recombinant M protein’s immunoreactivity with the sera from COVID-19 convalescents. In silico designed protein was created from the outer N-terminal part (19 aa) and internal C-terminal tail (101–222 aa) of the M protein (YP_009724393.1) and was recombinantly produced and purified. The designed M protein (16,498.74 Da, pI 8.79) revealed both IgM and IgG reactivity with serum samples from COVID-19 convalescents in Western blot. In ELISA, more than 93% (28/30) of COVID-19 sera were positive for IgM detection, and more than 96% (29/30) were positive for specific IgG detection to M protein. Based on the capacity to provoke an immune response and its strong antigenic properties, as shown here, and the fact that it is also involved in the virion entry into host cells, the M protein of the SARS-CoV-2 virus as a good antigen has the potential in diagnostic purposes and vaccine design. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/22/9/4951; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms22094951 |
| URL الوصول: | https://doaj.org/article/033e5971fcf94cceaac66b2ea3655d6a |
| رقم الأكسشن: | edsdoj.033e5971fcf94cceaac66b2ea3655d6a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms22094951 |