دورية أكاديمية
RADAR: differential analysis of MeRIP-seq data with a random effect model
| العنوان: | RADAR: differential analysis of MeRIP-seq data with a random effect model |
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| المؤلفون: | Zijie Zhang, Qi Zhan, Mark Eckert, Allen Zhu, Agnieszka Chryplewicz, Dario F. De Jesus, Decheng Ren, Rohit N. Kulkarni, Ernst Lengyel, Chuan He, Mengjie Chen |
| المصدر: | Genome Biology, Vol 20, Iss 1, Pp 1-17 (2019) |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Biology (General) LCC:Genetics |
| مصطلحات موضوعية: | N 6-adenosine methylation (m6A), Differential methylation, MeRIP-seq, Biology (General), QH301-705.5, Genetics, QH426-470 |
| الوصف: | Abstract Epitranscriptome profiling using MeRIP-seq is a powerful technique for in vivo functional studies of reversible RNA modifications. We develop RADAR, a comprehensive analytical tool for detecting differentially methylated loci in MeRIP-seq data. RADAR enables accurate identification of altered methylation sites by accommodating variability of pre-immunoprecipitation expression level and post-immunoprecipitation count using different strategies. In addition, it is compatible with complex study design when covariates need to be incorporated in the analysis. Through simulation and real dataset analyses, we show that RADAR leads to more accurate and reproducible differential methylation analysis results than alternatives, which is available at https://github.com/scottzijiezhang/RADAR. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1474-760X |
| Relation: | https://doaj.org/toc/1474-760X |
| DOI: | 10.1186/s13059-019-1915-9 |
| URL الوصول: | https://doaj.org/article/034a399bc5b94967bd10e5e307ab8404 |
| رقم الأكسشن: | edsdoj.034a399bc5b94967bd10e5e307ab8404 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1474760X |
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| DOI: | 10.1186/s13059-019-1915-9 |