دورية أكاديمية
Derivation of Human Extraembryonic Mesoderm-like Cells from Primitive Endoderm
| العنوان: | Derivation of Human Extraembryonic Mesoderm-like Cells from Primitive Endoderm |
|---|---|
| المؤلفون: | Karin Farkas, Elisabetta Ferretti |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 14, p 11366 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | extraembryonic mesoderm, primitive endoderm, hypoblast, yolk sac, vasculogenesis, placenta, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | In vitro modeling of human peri-gastrulation development is a valuable tool for understanding embryogenetic mechanisms. The extraembryonic mesoderm (ExM) is crucial in supporting embryonic development by forming tissues such as the yolk sac, allantois, and chorionic villi. However, the origin of human ExM remains only partially understood. While evidence suggests a primitive endoderm (PrE) origin based on morphological findings, current in vitro models use epiblast-like cells. To address this gap, we developed a protocol to generate ExM-like cells from PrE-like cell line called naïve extraembryonic endoderm (nEnd). We identified the ExM-like cells by specific markers (LUM and ANXA1). Moreover, these in vitro-produced ExM cells displayed angiogenic potential on a soft matrix, mirroring their physiological role in vasculogenesis. By integrating single-cell RNA sequencing (scRNAseq) data, we found that the ExM-like cells clustered with the LUM/ANXA1-rich cell populations of the gastrulating embryo, indicating similarity between in vitro and ex utero cell populations. This study confirms the derivation of ExM from PrE and establishes a cell culture system that can be utilized to investigate ExM during human peri-gastrulation development, both in monolayer cultures and more complex models. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/24/14/11366; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms241411366 |
| URL الوصول: | https://doaj.org/article/c03aa0aeaf61458c9a881ff1afc6e17f |
| رقم الأكسشن: | edsdoj.03aa0aeaf61458c9a881ff1afc6e17f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
|---|---|
| DOI: | 10.3390/ijms241411366 |