The advent of the Internet of Things (IoT) has led to embedding wireless transceivers into a wide range of devices, in order to implement context-aware scenarios, in which a massive amount of transceivers is foreseen. In this framework, cost-effective electronic and Radio Frequency (RF) front-end integration is desirable, in order to enable straightforward inclusion of communication capabilities within objects and devices in general. In this work, flexible antenna prototypes, based on screen-printing techniques, with conductive inks on flexible low-cost plastic substrates is proposed. Different parameters such as substrate/ink characteristics are considered, as well as variations in fabrication process or substrate angular deflection in device performance. Simulation and measurement results are presented, as well as system validation results in a real test environment in wireless sensor network communications. The results show the feasibility of using screen-printing antenna elements on flexible low-cost substrates, which can be embedded in a wide array of IoT scenarios.