دورية أكاديمية
Establishing a high sensitivity detection method for SARS-CoV-2 IgM/IgG and developing a clinical application of this method
| العنوان: | Establishing a high sensitivity detection method for SARS-CoV-2 IgM/IgG and developing a clinical application of this method |
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| المؤلفون: | Chunyan Zhang, Lei Zhou, Hao Liu, Sibing Zhang, Yaping Tian, Junli Huo, Fei Li, Yao Zhang, Bo Wei, Dan Xu, Jinwei Hu, Jiayi Wang, Yuxuan Cheng, Wenjie Shi, Xiuli Xu, Jianping Zhou, Peipei Sang, Xudong Tan, Weiwei Wang, Minjie Zhang, Bin Wang, Yujun Zhou, Kan Zhang, Kunlun He |
| المصدر: | Emerging Microbes and Infections, Vol 9, Iss 1, Pp 2020-2029 (2020) |
| بيانات النشر: | Taylor & Francis Group, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Infectious and parasitic diseases LCC:Microbiology |
| مصطلحات موضوعية: | SARS-CoV-2 antibody, multi-epitopes fusion protein, time-resolved fluorescence immunoassay, disease evaluation, prognosis, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502 |
| الوصف: | ABSTRACTCOVID-19 is caused by SARS-CoV-2 infection and was initially discovered in Wuhan. This outbreak quickly spread all over China and then to more than 20 other countries. SARS-CoV-2 fluorescent microsphere immunochromatographic test strips were prepared by the combination of time-resolved fluorescence immunoassay with a lateral flow assay. The analytical performance and clinical evaluation of this testing method was done and the clinical significance of the testing method was verified. The LLOD of SARS-CoV-2 antibody IgG and IgM was 0.121U/L and 0.366U/L. The specificity of IgM and IgG strips in healthy people and in patients with non-COVID-19 disease was 94%, 96.72% and 95.50%, 99.49%, respectively; and sensitivity of IgM and IgG strips for patients during treatment and follow-up was 63.02%, 37.61% and 87.28%, 90.17%, respectively. The SARS-CoV-2 antibody test strip can provide rapid, flexible and accurate testing, and is able to meet the clinical requirement for rapid on-site testing of virus. The ability to detect IgM and IgG provided a significant benefit for the detection and prediction of clinical course with COVID-19 patients. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 22221751 2222-1751 |
| Relation: | https://doaj.org/toc/2222-1751 |
| DOI: | 10.1080/22221751.2020.1811161 |
| URL الوصول: | https://doaj.org/article/051b51807b6c408aaa776073a87e28d5 |
| رقم الأكسشن: | edsdoj.051b51807b6c408aaa776073a87e28d5 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 22221751 |
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| DOI: | 10.1080/22221751.2020.1811161 |