دورية أكاديمية
Long-term administration of probiotics prevents gastrointestinal mucosal barrier dysfunction in septic mice partly by upregulating the 5-HT degradation pathway
| العنوان: | Long-term administration of probiotics prevents gastrointestinal mucosal barrier dysfunction in septic mice partly by upregulating the 5-HT degradation pathway |
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| المؤلفون: | Cao Xiaopeng, Zhao Hui, Liang Zhimin, Cao Yi, Min Min |
| المصدر: | Open Medicine, Vol 18, Iss 1, Pp 75-87 (2023) |
| بيانات النشر: | De Gruyter, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | sepsis, probiotics, gastrointestinal barrier, 5-hydroxytryptamine, erk signal, Medicine |
| الوصف: | Sepsis can impair gastrointestinal (GI) barrier integrity. Oral probiotics (PT) can maintain the balance of GI microflora and improve GI function. 5-Hydroxytryptamine (5-HT) is a key promoter of GI injury caused by sepsis. However, the mechanism by which PT attenuates sepsis by regulating 5-HT is not fully understood. In this study, C57BL6 mice were intragastric administrated with normal saline (NC) or PT once a day for 4 weeks before cecal ligation and puncture (CLP). Compared with NC-CLP mice, PT-CLP mice had lower clinical score, higher body temperature. The survival rate of PT-CLP mice was significantly improved. The levels of inflammatory cytokines and 5-HT were obviously decreased in PT-CLP mice, and GI peristalsis and barrier function were enhanced. Moreover, sepsis downregulated the expression of tight junction proteins, while PT pretreatment could maintain them at the level of sham operation group. Furthermore, PT pretreatment increased the expression of serotonin transporter and monoamine oxidase A. PT administration could inhibit NF-κB activity, and activate ERK activity. In conclusion, long-term supplementation of PT before CLP can prevent sepsis-induced GI mucosal barrier dysfunction in mice, which may be partially mediated by upregulating the 5-HT degradation pathway via activating ERK signaling. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2391-5463 2023-0869 |
| Relation: | https://doaj.org/toc/2391-5463 |
| DOI: | 10.1515/med-2023-0869 |
| URL الوصول: | https://doaj.org/article/05263e159c264457ac946c820bf5e3da |
| رقم الأكسشن: | edsdoj.05263e159c264457ac946c820bf5e3da |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23915463 20230869 |
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| DOI: | 10.1515/med-2023-0869 |