دورية أكاديمية
COVID-19 wastewater based epidemiology: long-term monitoring of 10 WWTP in France reveals the importance of the sampling context
| العنوان: | COVID-19 wastewater based epidemiology: long-term monitoring of 10 WWTP in France reveals the importance of the sampling context |
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| المؤلفون: | A. Lazuka, C. Arnal, E. Soyeux, M. Sampson, A.-S. Lepeuple, Y. Deleuze, S. Pouradier Duteil, S. Lacroix |
| المصدر: | Water Science and Technology, Vol 84, Iss 8, Pp 1997-2013 (2021) |
| بيانات النشر: | IWA Publishing, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Environmental technology. Sanitary engineering |
| مصطلحات موضوعية: | covid-19, public health, rt-qpcr, sampling context, sars-cov-2, wastewater-based epidemiology, Environmental technology. Sanitary engineering, TD1-1066 |
| الوصف: | SARS-CoV-2 wastewater-based epidemiology (WBE) has been advanced as a relevant indicator of distribution of COVID-19 in communities, supporting classical testing and tracing epidemiological approaches. An extensive sampling campaign, including ten municipal wastewater treatment plants, has been conducted in different cities of France over a 20-week period, encompassing the second peak of COVID-19 outbreak in France. A well-recognised ultrafiltration – RNA extraction – RT-qPCR protocol was used and qualified, showing 5.5 +/− 0.5% recovery yield on heat-inactivated SARS-CoV-2. Importantly the whole, solid and liquid, fraction of wastewater was used for virus concentration in this study. Campaign results showed medium- to strong- correlation between SARS-CoV-2 WBE data and COVID-19 prevalence. To go further, statistical relationships between WWTP inlet flow rate and rainfall were studied and taken into account for each WWTP in order to calculate contextualized SARS-CoV-2 loads. This metric presented improved correlation strengths with COVID-19 prevalence for WWTP particularly submitted and sensitive to rain. Such findings highlighted that SARS-CoV-2 WBE data ultimately require to be contextualized for relevant interpretation. HIGHLIGHTS First study monitoring inlet of 10 WWTPs located in France for SARS-CoV-2 RNA quantification over a 20-week period encompassing the second peak of COVID-19 outbreak.; Viral recovery yield was 5.5% +/− 0.5% using heat-inactivated SARS-CoV-2.; Medium to high Spearman's correlation strength was observed between SARS-CoV-2 WBE and COVID-19 prevalence data.; Considering sampling context (i.e. rain events) improved data consistency and correlation strength.; |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0273-1223 1996-9732 |
| Relation: | http://wst.iwaponline.com/content/84/8/1997; https://doaj.org/toc/0273-1223; https://doaj.org/toc/1996-9732 |
| DOI: | 10.2166/wst.2021.418 |
| URL الوصول: | https://doaj.org/article/d054455e8c8844c28a49879d5110f0c5 |
| رقم الأكسشن: | edsdoj.054455e8c8844c28a49879d5110f0c5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 02731223 19969732 |
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| DOI: | 10.2166/wst.2021.418 |