دورية أكاديمية
Rapid measurement of bacterial contamination in water: A catalase responsive-electrochemical sensor
| العنوان: | Rapid measurement of bacterial contamination in water: A catalase responsive-electrochemical sensor |
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| المؤلفون: | Arti Sharma, Akanksha Mishra, Meenu Chhabra |
| المصدر: | Heliyon, Vol 10, Iss 5, Pp e26724- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Science (General) LCC:Social sciences (General) |
| مصطلحات موضوعية: | Bacterial sensor, Potentiometric response, Coliforms, Hydrogen peroxide, Electrochemical reactions, Catalase reaction, Science (General), Q1-390, Social sciences (General), H1-99 |
| الوصف: | The present study describes the development of a potentiometric sensor for microbial monitoring in water based on catalase activity. The sensor comprises a MnO2-modified electrode that responds linearly to hydrogen peroxide (H2O2) from 0.16 M to 3.26 M. The electrode potential drops when the H2O2 solution is spiked with catalase or catalase-producing microorganisms that decompose H2O2. The sensor is responsive to different bacteria and their catalase activities. The electrochemical sensor exhibits a lower limit of detection (LOD) for Escherichia coli at 11 CFU/ml, Citrobacter youngae at 12 CFU/ml, and Pseudomonas aeruginosa at 23 CFU/ml. The sensor shows high sensitivity at 3.49, 3.02, and 4.24 mV/cm2dec for E. coli, C. youngae, and P. aeruginosa, respectively. The abiotic sensing electrode can be used multiple times without changing the response potential (up to 100 readings) with a shelf-life of over six months. The response time is a few seconds, with a total test time of 5 min. Additionally, the sensor effectively tested actual samples (drinking and grey water), which makes it a quick and reliable sensing tool. Therefore, the study offers a promising water monitoring tool with high sensitivity, stability, good detection limit, and minimum interference from other water contaminants. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-8440 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405844024027555; https://doaj.org/toc/2405-8440 |
| DOI: | 10.1016/j.heliyon.2024.e26724 |
| URL الوصول: | https://doaj.org/article/05c4e156ac5646fe9cb1525545269a21 |
| رقم الأكسشن: | edsdoj.05c4e156ac5646fe9cb1525545269a21 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24058440 |
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| DOI: | 10.1016/j.heliyon.2024.e26724 |