دورية أكاديمية
Subnormal GM1 in PBMCs: Promise for Early Diagnosis of Parkinson’s Disease?
| العنوان: | Subnormal GM1 in PBMCs: Promise for Early Diagnosis of Parkinson’s Disease? |
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| المؤلفون: | Samar K. Alselehdar, Monami Chakraborty, Suman Chowdhury, Roy N. Alcalay, Matthew Surface, Robert Ledeen |
| المصدر: | International Journal of Molecular Sciences, Vol 22, Iss 21, p 11522 (2021) |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | early diagnosis, sporadic Parkinson’s disease, GBA variant of Parkinson’s, GM1 ganglioside, GD1a ganglioside, PBMCs, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | The fact that Parkinson’s disease (PD) pathologies are well advanced in most PD patients by the time of clinical elucidation attests to the importance of early diagnosis. Our attempt to achieve this has capitalized on our previous finding that GM1 ganglioside is expressed at subnormal levels in virtually all tissues of sporadic PD (sPD) patients including blood cells. GM1 is present in most vertebrate cells, is especially abundant in neurons where it was shown essential for their effective functioning and long term viability. We have utilized peripheral blood mononuclear cells (PBMCs) which, despite their low GM1, we found to be significantly lower in sPD patients compared to age-matched healthy controls. To quantify GM1 (and GD1a) we used high performance thin-layer chromatography combined with cholera toxin B linked to horseradish peroxidase, followed by densitometric quantification. GM1 was also deficient in PBMCs from PD patients with mutations in the glucocerebrosidase gene (PD-GBA), apparently even lower than in sPD. Reasons are given why we believe these results obtained with patients manifesting fully developed PD will apply as well to PD patients in preclinical stages—a topic for future study. We also suggest that these findings point to a potential disease altering therapy for PD once the early diagnosis is established. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 22211152 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/22/21/11522; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms222111522 |
| URL الوصول: | https://doaj.org/article/a0606aed9acc4f3cac756aa733a7c410 |
| رقم الأكسشن: | edsdoj.0606aed9acc4f3cac756aa733a7c410 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22211152 14220067 16616596 |
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| DOI: | 10.3390/ijms222111522 |