دورية أكاديمية
أعرض في EDS

Differential lncRNA/mRNA expression profiling and ceRNA network analyses in amniotic fluid from foetuses with ventricular septal defects

التفاصيل البيبلوغرافية
العنوان: Differential lncRNA/mRNA expression profiling and ceRNA network analyses in amniotic fluid from foetuses with ventricular septal defects
المؤلفون: Huaming Wang, Xi Lin, Zecheng Wang, Shaozheng He, Bingtian Dong, Guorong Lyu
المصدر: PeerJ, Vol 11, p e14962 (2023)
بيانات النشر: PeerJ Inc., 2023.
سنة النشر: 2023
المجموعة: LCC:Medicine
LCC:Biology (General)
مصطلحات موضوعية: Long noncoding RNA, Ventricular septal defect, Competitive endogenous RNAs, Amniotic fluid, Prenatal diagnosis, Medicine, Biology (General), QH301-705.5
الوصف: Background Long noncoding RNAs (lncRNAs) have been shown to be involved in the regulation of numerous biological processes in embryonic development. We aimed to explore lncRNA expression profiles in ventricular septal defects (VSDs) and reveal their potential roles in heart development. Methods Microarray analyses were performed to screen differentially expressed lncRNAs (DE-lncRNAs) and mRNAs (DE-mRNAs) in the amniotic fluid between the VSD group and the control group. Bioinformatics analyses were further used to identify the functional enrichment and signaling pathways of important mRNAs. Then, a coding–noncoding gene coexpression (CNC) network and competitive endogenous RNAs (ceRNA) network were drawn. Finally, qRT‒PCR was performed to verify several hub lncRNAs and mRNAs in the network. Results A total of 710 DE-lncRNAs and 397 DE-mRNAs were identified in the VSD group. GO and KEGG analyses revealed that the DE-mRNAs were enriched in cardiac development-related biological processes and pathways, including cell proliferation, cell apoptosis, and the Sonic Hedgehog signaling pathway. Four VSD related mRNAs was used to construct the CNC network, which included 149 pairs of coexpressing lncRNAs and mRNAs. In addition, a ceRNA network, including 15 lncRNAs, 194 miRNAs, and four mRNAs, was constructed to reveal the potential regulatory relationship between lncRNAs and protein-coding genes. Finally, seven RNAs in the ceRNA network were validated, including IDS, NR2F2, GPC3, LINC00598, GATA3-AS1, PWRN1, and LINC01551. Conclusion Our study identified some lncRNAs and mRNAs may be potential biomarkers and therapeutic targets for foetuses with VSD, and described the lncRNA-associated ceRNA network in the progression of VSD.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2167-8359
Relation: https://peerj.com/articles/14962.pdf; https://peerj.com/articles/14962/; https://doaj.org/toc/2167-8359
DOI: 10.7717/peerj.14962
URL الوصول: https://doaj.org/article/0608a7ec6ffe478f8bb3832bd4fb1a58
رقم الأكسشن: edsdoj.0608a7ec6ffe478f8bb3832bd4fb1a58
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:21678359
DOI:10.7717/peerj.14962