دورية أكاديمية
Establishment of a pancreatic cancer animal model using the pancreas-targeted hydrodynamic gene delivery method
| العنوان: | Establishment of a pancreatic cancer animal model using the pancreas-targeted hydrodynamic gene delivery method |
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| المؤلفون: | Osamu Shibata, Kenya Kamimura, Yuto Tanaka, Kohei Ogawa, Takashi Owaki, Chiyumi Oda, Shinichi Morita, Atsushi Kimura, Hiroyuki Abe, Satoshi Ikarashi, Kazunao Hayashi, Takeshi Yokoo, Shuji Terai |
| المصدر: | Molecular Therapy: Nucleic Acids, Vol 28, Iss , Pp 342-352 (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | delivery strategies, pancreatic cancer, animal model, gene delivery, hydrodynamic gene delivery, oncogene, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | This research developed an easy-to-use, reproducible pancreatic cancer animal model utilizing pancreas-targeted hydrodynamic gene delivery to deliver human pancreatic cancer-related genes to the pancreas of wild-type rats. KRASG12D-induced pancreatic intraepithelial neoplasia lesions showed malignant transformation in the main pancreatic duct at 4 weeks and developed acinar-to-ductal metaplasia, which led to pancreatic ductal adenocarcinoma within 5 weeks, and the gene combination of KRASG12D and YAP enhanced these effects. The repeat hydrodynamic gene delivery of KRASG12D + YAP combination at 4 weeks showed acinar-to-ductal metaplasia in all rats and pancreatic ductal adenocarcinoma in 80% of rats 1 week later. Metastatic tumors in the liver, lymph nodes, and subcutaneous lesions and nervous invasion were confirmed. KRASG12D and YAP combined transfer contributes to the E- to N-cadherin switch in pancreatic ductal adenocarcinoma cells and to tumor metastases. This pancreatic cancer model will speed up pancreatic cancer research for novel treatments and biomarkers for early diagnosis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2162-2531 29951046 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2162253122000683; https://doaj.org/toc/2162-2531 |
| DOI: | 10.1016/j.omtn.2022.03.019 |
| URL الوصول: | https://doaj.org/article/06496c299510468bace0c2f71ac60a07 |
| رقم الأكسشن: | edsdoj.06496c299510468bace0c2f71ac60a07 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21622531 29951046 |
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| DOI: | 10.1016/j.omtn.2022.03.019 |