دورية أكاديمية
Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers
| العنوان: | Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers |
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| المؤلفون: | Denise Zujur, Kosuke Kanke, Shoko Onodera, Shoichiro Tani, Jenny Lai, Toshifumi Azuma, Xiaonan Xin, Alexander C. Lichtler, David W. Rowe, Taku Saito, Sakae Tanaka, Hideki Masaki, Hiromitsu Nakauchi, Ung-il Chung, Hironori Hojo, Shinsuke Ohba |
| المصدر: | Regenerative Therapy, Vol 14, Iss , Pp 19-31 (2020) |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine (General) LCC:Cytology |
| مصطلحات موضوعية: | Mesoderm, Wnt, Hedgehog, Helioxanthin, Three-dimensional culture, Medicine (General), R5-920, Cytology, QH573-671 |
| الوصف: | Clinically relevant human induced pluripotent stem cell (hiPSC) derivatives require efficient protocols to differentiate hiPSCs into specific lineages. Here we developed a fully defined xeno-free strategy to direct hiPSCs toward osteoblasts within 21 days. The strategy successfully achieved the osteogenic induction of four independently derived hiPSC lines by a sequential use of combinations of small-molecule inducers. The induction first generated mesodermal cells, which subsequently recapitulated the developmental expression pattern of major osteoblast genes and proteins. Importantly, Col2.3-Cherry hiPSCs subjected to this strategy strongly expressed the cherry fluorescence that has been observed in bone-forming osteoblasts in vivo. Moreover, the protocol combined with a three-dimensional (3D) scaffold was suitable for the generation of a xeno-free 3D osteogenic system. Thus, our strategy offers a platform with significant advantages for bone biology studies and it will also contribute to clinical applications of hiPSCs to skeletal regenerative medicine. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2352-3204 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2352320419301683; https://doaj.org/toc/2352-3204 |
| DOI: | 10.1016/j.reth.2019.12.010 |
| URL الوصول: | https://doaj.org/article/06a2c4f04f66498f94f1a291761848bf |
| رقم الأكسشن: | edsdoj.06a2c4f04f66498f94f1a291761848bf |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23523204 |
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| DOI: | 10.1016/j.reth.2019.12.010 |