دورية أكاديمية
Combining combing and secondary ion mass spectrometry to study DNA on chips using 13C and 15N labeling [version 1; referees: 2 approved]
العنوان: | Combining combing and secondary ion mass spectrometry to study DNA on chips using 13C and 15N labeling [version 1; referees: 2 approved] |
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المؤلفون: | Armelle Cabin-Flaman, Anne-Francoise Monnier, Yannick Coffinier, Jean-Nicolas Audinot, David Gibouin, Tom Wirtz, Rabah Boukherroub, Henri-Noël Migeon, Aaron Bensimon, Laurent Jannière, Camille Ripoll, Victor Norris |
المصدر: | F1000Research, Vol 5 (2016) |
بيانات النشر: | F1000 Research Ltd, 2016. |
سنة النشر: | 2016 |
المجموعة: | LCC:Medicine LCC:Science |
مصطلحات موضوعية: | Structure: Transcription & Translation, Medicine, Science |
الوصف: | Dynamic secondary ion mass spectrometry (D-SIMS) imaging of combed DNA – the combing, imaging by SIMS or CIS method – has been developed previously using a standard NanoSIMS 50 to reveal, on the 50 nm scale, individual DNA fibers labeled with different, non-radioactive isotopes in vivo and to quantify these isotopes. This makes CIS especially suitable for determining the times, places and rates of DNA synthesis as well as the detection of the fine-scale re-arrangements of DNA and of molecules associated with combed DNA fibers. Here, we show how CIS may be extended to 13C-labeling via the detection and quantification of the 13C14N- recombinant ion and the use of the 13C:12C ratio, we discuss how CIS might permit three successive labels, and we suggest ideas that might be explored using CIS. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2046-1402 18620086 |
Relation: | http://f1000research.com/articles/5-1437/v1; https://doaj.org/toc/2046-1402 |
DOI: | 10.12688/f1000research.8361.1 |
URL الوصول: | https://doaj.org/article/06afbbafed934cfe9a186200866d7457 |
رقم الأكسشن: | edsdoj.06afbbafed934cfe9a186200866d7457 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 20461402 18620086 |
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DOI: | 10.12688/f1000research.8361.1 |