دورية أكاديمية

Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids

التفاصيل البيبلوغرافية
العنوان: Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids
المؤلفون: Fernanda Motta da Costa Santos, Giovana Augusta Torres, Vânia Helena Techio, Antônio Vander Pereira, Lisete Chamma Davide
المصدر: Pesquisa Agropecuária Brasileira, Vol 52, Iss 9, Pp 814-817 (2017)
بيانات النشر: Embrapa Informação Tecnológica, 2017.
سنة النشر: 2017
المجموعة: LCC:Agriculture (General)
مصطلحات موضوعية: Pennisetum glaucum, Pennisetum purpureum, in situ hybridization, intergenomic pairing, interspecific hybridization, Agriculture (General), S1-972
الوصف: Abstract: The objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
Spanish; Castilian
Portuguese
تدمد: 1678-3921
0100-204x
Relation: http://www.scielo.br/pdf/pab/v52n9/1678-3921-pab-52-09-00814.pdf; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000900814&tlng=en; https://doaj.org/toc/1678-3921
DOI: 10.1590/s0100-204x2017000900014
URL الوصول: https://doaj.org/article/075c1bacfa3a487d823f8f3f1643f5a9
رقم الأكسشن: edsdoj.075c1bacfa3a487d823f8f3f1643f5a9
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:16783921
0100204x
DOI:10.1590/s0100-204x2017000900014