دورية أكاديمية
Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids
العنوان: | Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids |
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المؤلفون: | Fernanda Motta da Costa Santos, Giovana Augusta Torres, Vânia Helena Techio, Antônio Vander Pereira, Lisete Chamma Davide |
المصدر: | Pesquisa Agropecuária Brasileira, Vol 52, Iss 9, Pp 814-817 (2017) |
بيانات النشر: | Embrapa Informação Tecnológica, 2017. |
سنة النشر: | 2017 |
المجموعة: | LCC:Agriculture (General) |
مصطلحات موضوعية: | Pennisetum glaucum, Pennisetum purpureum, in situ hybridization, intergenomic pairing, interspecific hybridization, Agriculture (General), S1-972 |
الوصف: | Abstract: The objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English Spanish; Castilian Portuguese |
تدمد: | 1678-3921 0100-204x |
Relation: | http://www.scielo.br/pdf/pab/v52n9/1678-3921-pab-52-09-00814.pdf; http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000900814&tlng=en; https://doaj.org/toc/1678-3921 |
DOI: | 10.1590/s0100-204x2017000900014 |
URL الوصول: | https://doaj.org/article/075c1bacfa3a487d823f8f3f1643f5a9 |
رقم الأكسشن: | edsdoj.075c1bacfa3a487d823f8f3f1643f5a9 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 16783921 0100204x |
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DOI: | 10.1590/s0100-204x2017000900014 |