دورية أكاديمية
Metabolic engineering of Escherichia coli for efficient degradation of 4-fluorophenol
| العنوان: | Metabolic engineering of Escherichia coli for efficient degradation of 4-fluorophenol |
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| المؤلفون: | Lijuan Wang, Rihe Peng, Yongsheng Tian, Jing Xu, Bo Wang, Hongjuan Han, Xiaoyan Fu, Jianjie Gao, Quanhong Yao |
| المصدر: | AMB Express, Vol 12, Iss 1, Pp 1-10 (2022) |
| بيانات النشر: | SpringerOpen, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biotechnology LCC:Microbiology |
| مصطلحات موضوعية: | Multi-monocistronic, 4-Fluorophenol, β-Ketoadipate, Degradation, 4-Substituted phenol, Biotechnology, TP248.13-248.65, Microbiology, QR1-502 |
| الوصف: | Abstract As a kind of refractory organic pollutant, 4-fluorophenol (4-FP) can be degraded by only a few microorganisms with low efficiency because of the great electron-withdrawing ability of fluorine atoms. So it is necessary to artificially construct engineered strain to improve the degradation efficiency and meet the requirements of pollutant degradation. In this study, four genes (fpdA2, fpdB, fpdC, and fpdD) for 4-FP degradation from Arthrobacter sp. strain IF1 were optimized and synthesized and then reconstructed into Escherichia coli by a multi-monocistronic vector to obtain recombinant BL-fpd that could degrade 4-FP efficiently. Under optimized induction conditions (inducing the strain by 2 g/L L-arabinose and 1 mM IPTG at 37 ℃), BL-fpd could completely degrade 2 mM 4-FP, 4-chlorophenol, 4-bromophenol, and 4-nitrophenol into β-ketoadipate, which could be further metabolized by the bacteria. FpdA2 showed the highest activity towards 4-bromophenol. The strain could completely degrade 1 mM 4-FP in industrial wastewater within 3 h. This study provided a promising strain for the degradation of 4-FP and some other 4-substituted phenols. The construction technologies of multi-monocistronic expression vector may also be used to construct other organic pollutants degrading bacteria. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2191-0855 |
| Relation: | https://doaj.org/toc/2191-0855 |
| DOI: | 10.1186/s13568-022-01396-9 |
| URL الوصول: | https://doaj.org/article/076d522ed7e348578c51106eb387946b |
| رقم الأكسشن: | edsdoj.076d522ed7e348578c51106eb387946b |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 21910855 |
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| DOI: | 10.1186/s13568-022-01396-9 |