دورية أكاديمية
Spatio-temporal clustering of African swine fever virus (Asfarviridae: Asfivirus) circulating in the Kaliningrad region based on three genome markers
| العنوان: | Spatio-temporal clustering of African swine fever virus (Asfarviridae: Asfivirus) circulating in the Kaliningrad region based on three genome markers |
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| المؤلفون: | Roman S. Chernyshev, Alexey S. Igolkin, Andrey R. Shotin, Nikolay G. Zinyakov, Ivan S. Kolbin, Anastasia S. Sadchikova, Ivan A. Lavrentiev, Konstantin N. Gruzdev, Ali Mazloum |
| المصدر: | Вопросы вирусологии, Vol 69, Iss 3, Pp 241-254 (2024) |
| بيانات النشر: | Central Research Institute for Epidemiology, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | african swine fever, genome markers of spread, spatio-temporal analysis, genetic variants, kaliningrad region, Microbiology, QR1-502 |
| الوصف: | Introduction. The rapid spread of African swine fever in the Kaliningrad region makes it necessary to use the methods of molecular epidemiology to determine the dynamics and direction of ASF spread in this region of Russia. The aim of the study was to determine single nucleotide polymorphisms within molecular markers K145R, O174L and MGF 505-5R of ASFVs isolated in Kaliningrad region and to study the circulating of the pathogen in European countries by subgenotyping and spatio-temporal clustering analysis. Materials and methods. Blood samples from living domestic pigs and organs from dead domestic pigs and wild boars, collected in the Kaliningrad region between 2017 and 2022 were used. Virus isolation was carried out in porcine bone-marrow primary cell culture. Amplicons of genome markers were amplified by PCR with electrophoretic detection and subsequent extraction of fragments from agarose gel. Sequencing was performed using the Sanger method. Results. The circulation of two genetic clusters of ASFV isolates on the territory of the Kaliningrad has been established: epidemic (K145R-III, MGF 505-5R-II, O174L-I – 94.3% of the studied isolates) and sporadic (K145R-II, MGF 505-5R-II, O174L-I – 5.7%). Conclusion. The broaden molecular genetic surveillance of ASFV isolates based on sequencing of genome markers is necessary in the countries of the Eurasian continent to perform a more detailed analysis of ASF spread between countries and within regions. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Russian |
| تدمد: | 0507-4088 2411-2097 |
| Relation: | https://virusjour.crie.ru/jour/article/viewFile/16624/893; https://doaj.org/toc/0507-4088; https://doaj.org/toc/2411-2097 |
| DOI: | 10.36233/0507-4088-231 |
| URL الوصول: | https://doaj.org/article/078f2cb903c94b3b98d55a25125a509d |
| رقم الأكسشن: | edsdoj.078f2cb903c94b3b98d55a25125a509d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 05074088 24112097 |
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| DOI: | 10.36233/0507-4088-231 |