دورية أكاديمية
A Novel Apoptosis Correlated Molecule: Expression and Characterization of Protein Latcripin-1 from Lentinula edodes C91–3
| العنوان: | A Novel Apoptosis Correlated Molecule: Expression and Characterization of Protein Latcripin-1 from Lentinula edodes C91–3 |
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| المؤلفون: | Min Huang, Wei Zhang, Lei Liu, Jing Cao, Anhong Ning, Yongzhi Lun, Xiaoli Wang, Wenchang Sun, Xingyun Li, Mintao Zhong, Ben Liu |
| المصدر: | International Journal of Molecular Sciences, Vol 13, Iss 5, Pp 6246-6265 (2012) |
| بيانات النشر: | MDPI AG, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | Lentinula edodes, apoptosis, protein Latcripin-1, Pichia pastoris, expression, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | An apoptosis correlated molecule—protein Latcripin-1 of Lentinula edodes C91-3—was expressed and characterized in Pichia pastoris GS115. The total RNA was obtained from Lentinula edodes C91–3. According to the transcriptome, the full-length gene of Latcripin-1 was isolated with 3'-Full Rapid Amplification of cDNA Ends (RACE) and 5'-Full RACE methods. The full-length gene was inserted into the secretory expression vector pPIC9K. The protein Latcripin-1 was expressed in Pichia pastoris GS115 and analyzed by Sodium Dodecylsulfonate Polyacrylate Gel Electrophoresis (SDS-PAGE) and Western blot. The Western blot showed that the protein was expressed successfully. The biological function of protein Latcripin-1 on A549 cells was studied with flow cytometry and the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyl-tetrazolium Bromide (MTT) method. The toxic effect of protein Latcripin-1 was detected with the MTT method by co-culturing the characterized protein with chick embryo fibroblasts. The MTT assay results showed that there was a great difference between protein Latcripin-1 groups and the control group (p < 0.05). There was no toxic effect of the characterized protein on chick embryo fibroblasts. The flow cytometry showed that there was a significant difference between the protein groups of interest and the control group according to apoptosis function (p < 0.05). At the same time, cell ultrastructure observed by transmission electron microscopy supported the results of flow cytometry. The work demonstrates that protein Latcripin-1 can induce apoptosis of human lung cancer cells A549 and brings new insights into and advantages to finding anti-tumor proteins. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 |
| Relation: | http://www.mdpi.com/1422-0067/13/5/6246; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms13056246 |
| URL الوصول: | https://doaj.org/article/07f0fc0a01d44668901060ef39a1ca1d |
| رقم الأكسشن: | edsdoj.07f0fc0a01d44668901060ef39a1ca1d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 |
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| DOI: | 10.3390/ijms13056246 |